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Neural cell integration into 3D bioprinted skeletal muscle constructs accelerates restoration of muscle function.
Nature Communications ( IF 14.7 ) Pub Date : 2020-02-24 , DOI: 10.1038/s41467-020-14930-9 Ji Hyun Kim 1 , Ickhee Kim 1 , Young-Joon Seol 1 , In Kap Ko 1 , James J Yoo 1 , Anthony Atala 1 , Sang Jin Lee 1
Nature Communications ( IF 14.7 ) Pub Date : 2020-02-24 , DOI: 10.1038/s41467-020-14930-9 Ji Hyun Kim 1 , Ickhee Kim 1 , Young-Joon Seol 1 , In Kap Ko 1 , James J Yoo 1 , Anthony Atala 1 , Sang Jin Lee 1
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A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration.
中文翻译：
将神经细胞整合到3D生物打印的骨骼肌构造中，可加速肌肉功能的恢复。
模仿天然骨骼肌的结构和功能特征的生物工程化骨骼肌构造是治疗广泛的肌肉缺陷损伤的有前途的治疗选择。我们以前表明，生物打印的人类骨骼肌构造能够形成具有对齐的肌纤维的多层束。在这项研究中，我们调查了神经细胞整合到生物打印的骨骼肌结构中以加速体内功能性肌肉再生的作用。神经输入到该生物打印的骨骼肌结构中显示了体外肌纤维形成，长期存活和神经肌肉接头形成的改善。更重要的是，具有神经细胞整合功能的生物印迹构建体有助于快速神经支配并成熟为有组织的肌肉组织，从而在肌肉缺损性损伤的啮齿动物模型中恢复正常的肌肉重量和功能。这些结果表明，可以将3D生物打印的人神经骨骼肌肉构造物与宿主神经网络快速整合，从而加速肌肉功能的恢复。
更新日期：2020-02-24
中文翻译：
将神经细胞整合到3D生物打印的骨骼肌构造中，可加速肌肉功能的恢复。
模仿天然骨骼肌的结构和功能特征的生物工程化骨骼肌构造是治疗广泛的肌肉缺陷损伤的有前途的治疗选择。我们以前表明，生物打印的人类骨骼肌构造能够形成具有对齐的肌纤维的多层束。在这项研究中，我们调查了神经细胞整合到生物打印的骨骼肌结构中以加速体内功能性肌肉再生的作用。神经输入到该生物打印的骨骼肌结构中显示了体外肌纤维形成，长期存活和神经肌肉接头形成的改善。更重要的是，具有神经细胞整合功能的生物印迹构建体有助于快速神经支配并成熟为有组织的肌肉组织，从而在肌肉缺损性损伤的啮齿动物模型中恢复正常的肌肉重量和功能。这些结果表明，可以将3D生物打印的人神经骨骼肌肉构造物与宿主神经网络快速整合，从而加速肌肉功能的恢复。
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