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Investigation of the active components and mechanisms of Schisandra chinensis in the treatment of asthma based on a network pharmacology approach and experimental validation.
Food & Function ( IF 5.1 ) Pub Date : 2020-04-30 , DOI: 10.1039/d0fo00087f
Xi Lv 1 , Zhiying Xu 1 , Guangyu Xu 1 , He Li 1 , Chunmei Wang 1 , Jianguang Chen 1 , Jinghui Sun 1
Food & Function ( IF 5.1 ) Pub Date : 2020-04-30 , DOI: 10.1039/d0fo00087f
Xi Lv 1 , Zhiying Xu 1 , Guangyu Xu 1 , He Li 1 , Chunmei Wang 1 , Jianguang Chen 1 , Jinghui Sun 1
Affiliation
The aim of this paper was to investigate the active components of Schisandra chinensis in the treatment of asthma and the related mechanisms by a network pharmacology approach. The active components of Schisandra chinensis and the corresponding targets were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Eight active components in Schisandra chinensis and 56 related targets were screened out according to two indicators, oral bioavailability (OB) and drug-likeness (DL). A total of 132 targets related to asthma were screened out through Therapeutic Target Database (TTD) data. The String database and Cytoscape software were used to build the "drug-active compound-target" network and protein-protein interaction (PPI) network. The key targets were further predicted by the analysis of related biological processes and the pathway-enrichment. A total of 10 intersection targets between Schisandra chinensis and asthma were obtained by building Venn diagrams, and lignans in Schisandra chinensis were found to be associated with asthma. The key targets Ptgs2 and Nos2 were further screened out, and schisandrol B (SCB) was predicted as the most related key component to asthma. A mouse asthma model was established with ovalbumin and aluminum hydroxide for verifying the effect of SCB and related mechanisms. The results showed that SCB could inhibit the gene expression of proinflammatory factors to play a therapeutic role in asthma by reducing the expression of Nos2 and Ptgs2 and regulating the NF-κB signaling pathway to intervene in the process of cell metabolism in mice. These results suggest that SCB can alleviate the severity of asthma through the mechanisms predicted by network pharmacology, and provide a basis for further understanding of the application of Schisandra chinensis in the treatment of asthma.
中文翻译:
基于网络药理学方法和实验验证,研究五味子治疗哮喘的活性成分和机理。
本文旨在通过网络药理学方法研究五味子在哮喘治疗中的活性成分及其相关机制。五味子的活性成分和相应的靶标是从中药系统药理数据库和分析平台(TCMSP)获得的。根据口服生物利用度(OB)和药物相似性(DL)这两个指标,筛选出五味子中的八个活性成分和56个相关目标。通过治疗目标数据库(TTD)数据筛选出总共132个与哮喘相关的目标。使用String数据库和Cytoscape软件来构建“药物活性化合物-靶标”网络和蛋白质-蛋白质相互作用(PPI)网络。通过相关生物学过程和途径富集的分析进一步预测了关键目标。通过建立维恩图,共获得五味子与哮喘之间的10个交集目标,发现五味子中的木脂素与哮喘有关。进一步筛选出关键目标Ptgs2和Nos2,并预测五味子乙(SCB)是哮喘最相关的关键成分。用卵清蛋白和氢氧化铝建立了小鼠哮喘模型,以验证SCB的作用和相关机制。结果表明,SCB可通过减少Nos2和Ptgs2的表达并调节NF-κB信号通路干预小鼠的细胞代谢过程,从而抑制促炎因子的基因表达,从而起到治疗哮喘的作用。
更新日期:2020-02-24
中文翻译:
基于网络药理学方法和实验验证,研究五味子治疗哮喘的活性成分和机理。
本文旨在通过网络药理学方法研究五味子在哮喘治疗中的活性成分及其相关机制。五味子的活性成分和相应的靶标是从中药系统药理数据库和分析平台(TCMSP)获得的。根据口服生物利用度(OB)和药物相似性(DL)这两个指标,筛选出五味子中的八个活性成分和56个相关目标。通过治疗目标数据库(TTD)数据筛选出总共132个与哮喘相关的目标。使用String数据库和Cytoscape软件来构建“药物活性化合物-靶标”网络和蛋白质-蛋白质相互作用(PPI)网络。通过相关生物学过程和途径富集的分析进一步预测了关键目标。通过建立维恩图,共获得五味子与哮喘之间的10个交集目标,发现五味子中的木脂素与哮喘有关。进一步筛选出关键目标Ptgs2和Nos2,并预测五味子乙(SCB)是哮喘最相关的关键成分。用卵清蛋白和氢氧化铝建立了小鼠哮喘模型,以验证SCB的作用和相关机制。结果表明,SCB可通过减少Nos2和Ptgs2的表达并调节NF-κB信号通路干预小鼠的细胞代谢过程,从而抑制促炎因子的基因表达,从而起到治疗哮喘的作用。