Facile synthesis of red dual-emissive carbon dots for ratiometric fluorescence sensing and cellular imaging.,Nanoscale

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Facile synthesis of red dual-emissive carbon dots for ratiometric fluorescence sensing and cellular imaging.
Nanoscale ( IF 5.8 ) Pub Date : 2020-02-24 , DOI: 10.1039/d0nr00381f
Yang Hu ₁ , Zhengbiao Yang ₂ , Xuan Lu ₁ , Jiazhuang Guo ₁ , Rui Cheng ₁ , Liangliang Zhu ₁ , Cai-Feng Wang ₁ , Su Chen ₁

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Recently, widespread attention has been paid to red emissive carbon dots (CDs) which have desirable optical properties, low toxicity, and biocompatibility. Despite great efforts, the facile preparation of red dual-emissive CDs useful for ratiometric detection and bioimaging remains challenging. Here, we report a facile synthesis of red dual-emissive CDs and their potential for ratiometric fluorescence sensing and cellular imaging. Derived from the hydrothermal treatment of dicyandiamide and o-phenylenediamine in dilute sulfuric acid, the CDs are surface-tailored with nitrogen-, oxygen-, and sulfur-containing functional groups. The as-prepared CDs show various good features, including good water solubility, biocompatibility, excitation-independent dual-emission with two photoluminescence (PL) peaks centered at 630 and 680 nm, and an absolute quantum yield (QY) of 30.2% in water. The CDs exhibit a selective, sensitive, rapid, and stable ratiometric fluorescence response toward methyl blue, giving a linear relationship in the range of 0.5-300 μM with a correlation coefficient (R2) of 0.997. We also study ratiometric fluorescence sensing for the accurate detection of pH. Moreover, the CDs possess good cellular imaging ability, indicating their promising applicability for biomedical applications. These results pave a way toward the fabrication of red dual-emissive carbon-based nanomaterials useful for both ratiometric sensing and bioimaging.

中文翻译：

简便的红色双发射碳点合成，用于比例荧光传感和细胞成像。

近来，已经广泛关注具有期望的光学性质，低毒性和生物相容性的红色发射碳点（CD）。尽管付出了巨大的努力，但如何简便地制备用于比率检测和生物成像的红色双发射CD仍然具有挑战性。在这里，我们报告了红色双发射CD的简便合成及其用于比率荧光传感和细胞成像的潜力。从双氰胺和邻苯二胺在稀硫酸中的水热处理获得，CD的表面具有含氮，氧和硫的官能团。所制备的CD表现出各种良好的功能，包括良好的水溶性，生物相容性，独立于激发的双重发射以及两个以630和680 nm为中心的光致发光（PL）峰，在水中的绝对量子产率（QY）为30.2％。CD表现出对甲基蓝的选择性，灵敏，快速和稳定的配比荧光响应，线性关系为0.5-300μM，相关系数（R2）为0.997。我们还研究了比例荧光传感技术，以精确检测pH值。此外，CD具有良好的细胞成像能力，表明它们在生物医学应用中具有广阔的应用前景。这些结果为制造可用于比率传感和生物成像的红色双发射碳基纳米材料铺平了道路。5-300μM，相关系数（R2）为0.997。我们还研究了比例荧光传感技术，以精确检测pH值。此外，CD具有良好的细胞成像能力，表明它们在生物医学应用中具有广阔的应用前景。这些结果为制造可用于比率传感和生物成像的红色双发射碳基纳米材料铺平了道路。5-300μM，相关系数（R2）为0.997。我们还研究了比例荧光传感技术，以精确检测pH值。此外，CD具有良好的细胞成像能力，表明它们在生物医学应用中具有广阔的应用前景。这些结果为制造可用于比率传感和生物成像的红色双发射碳基纳米材料铺平了道路。

更新日期：2020-03-05

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