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On-PKS Baeyer-Villiger-Type O-Atom Insertion Catalyzed by Luciferase-Like Monooxygenase OvmO during Olimycin Biosynthesis.
Organic Letters ( IF 4.9 ) Pub Date : 2020-02-19 , DOI: 10.1021/acs.orglett.0c00076 Chunyan Zhang 1, 2 , Huaran Zhang 2, 3 , Jianhua Ju 2, 3
Organic Letters ( IF 4.9 ) Pub Date : 2020-02-19 , DOI: 10.1021/acs.orglett.0c00076 Chunyan Zhang 1, 2 , Huaran Zhang 2, 3 , Jianhua Ju 2, 3
Affiliation
A silent ansamycin biosynthetic gene cluster (ovm) was activated in Streptomyces olivaceus SCSIO T05 following mutagenesis and media optimization. A new shunt product, olimycin C (1a) was produced by the ovmO-inactivated mutant strain, along with a minor product, olimycin D (1b). The production of these linear olimycin counterparts suggest that luciferase-like monooxygenase (LLM) OvmO catalyzes an on-PKS Baeyer-Villiger-type oxidation during assembly of the olimycin A (2) linear polyketide backbone.
中文翻译:
在奥林霉素生物合成过程中,萤光素酶样单加氧酶OvmO催化PKS上的Baeyer-Villiger型O原子插入。
诱变和培养基优化后,在橄榄链霉菌SCSIO T05中激活了沉默的安沙霉素生物合成基因簇(ovm)。ovmO灭活的突变菌株产生了新的分流产物奥霉素C(1a),次要产物奥霉素D(1b)。这些线性奥霉素对应物的产生表明,在荧光素A(2)线性聚酮骨架的组装过程中,萤光素酶样单加氧酶(LLM)OvmO催化On-PKS Baeyer-Villiger型氧化。
更新日期:2020-02-19
中文翻译:
在奥林霉素生物合成过程中,萤光素酶样单加氧酶OvmO催化PKS上的Baeyer-Villiger型O原子插入。
诱变和培养基优化后,在橄榄链霉菌SCSIO T05中激活了沉默的安沙霉素生物合成基因簇(ovm)。ovmO灭活的突变菌株产生了新的分流产物奥霉素C(1a),次要产物奥霉素D(1b)。这些线性奥霉素对应物的产生表明,在荧光素A(2)线性聚酮骨架的组装过程中,萤光素酶样单加氧酶(LLM)OvmO催化On-PKS Baeyer-Villiger型氧化。