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Modulating the properties of flow-assembled chitosan membranes in microfluidics with glutaraldehyde crosslinking.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-03-03 , DOI: 10.1039/c9tb02527h Piao Hu 1 , Christopher B Raub 2 , John S Choy 3 , Xiaolong Luo 1
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-03-03 , DOI: 10.1039/c9tb02527h Piao Hu 1 , Christopher B Raub 2 , John S Choy 3 , Xiaolong Luo 1
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Flow-assembled chitosan membranes are robust and semipermeable hydrogel structures formed in microfluidic devices that have been used for important applications such as gradient generation and studying cell-cell signaling. One challenge, however, remains unresolved. When a polydimethylsiloxane (PDMS) microchannel with a flow-assembled, deprotonated chitosan membrane (DCM) is treated with anti-adhesion agents such as Pluronic F-127 to prevent biomolecular and cellular adsorption on PDMS, the interaction between DCM and PDMS is compromised and the DCM easily delaminates. To address this challenge, DCMs in microfluidics are crosslinked with glutaraldehyde to modulate their properties, and the altered properties of the glutaraldehyde treated chitosan membrane (GTCM) are investigated. First, the GTCM's acidic resistance was confirmed, its mechanical robustness against hydrostatic pressure was significantly improved, and it remained intact on PDMS after Pluronic treatment. Second, crystallization in DCM and GTCM was investigated with quantitative polarized light microscopy (qPLM), which revealed that GTCM's optical retardance and anisotropy were lower, implying less molecular alignment than in DCM. Finally, membrane permeability was tested with FITC-labeled dextran transport experiments, which showed that the transport across GTCM was slightly higher than that across DCM. Overall, glutaraldehyde-crosslinked chitosan membrane has better acidic resistance, higher strength under Pluronic treatment, and less molecular microalignment, while its semi-permeability is retained. This study demonstrates how glutaraldehyde crosslinking can be used to modify and improve biopolymer membrane properties for broader applications, such as in an acidic environment or when Pluronic passivation is needed.
中文翻译：
戊二醛交联在微流控中调节流动组装的壳聚糖膜的性能。
流动组装的壳聚糖膜是在微流体装置中形成的坚固且半渗透的水凝胶结构，已用于重要应用，例如梯度产生和研究细胞信号传导。然而，一项挑战仍未解决。当将具有流动组装的去质子化壳聚糖膜（DCM）的聚二甲基硅氧烷（PDMS）微通道用抗粘剂（例如Pluronic F-127）处理以防止生物分子和细胞吸附在PDMS上时，DCM和PDMS之间的相互作用会受到损害，并且DCM容易分层。为了解决这一挑战，微流体中的DCM与戊二醛交联以调节其性能，并研究了戊二醛处理的壳聚糖膜（GTCM）的性能变化。首先，确认了GTCM的耐酸性，其抗静水压力的机械强度显着提高，经过Pluronic处理后，仍可在PDMS上保持完整。其次，使用定量偏振光显微镜（qPLM）研究了DCM和GTCM中的结晶，结果表明GTCM的光学延迟和各向异性较低，这意味着分子排列比DCM中的少。最后，通过FITC标记的葡聚糖转运实验对膜的渗透性进行了测试，结果表明，跨GTCM的转运略高于跨DCM的转运。总体而言，戊二醛交联的壳聚糖膜具有更好的耐酸性，在Pluronic处理下具有更高的强度，并且分子微排列更少，同时保留了半渗透性。
更新日期：2020-03-26
中文翻译：
戊二醛交联在微流控中调节流动组装的壳聚糖膜的性能。
流动组装的壳聚糖膜是在微流体装置中形成的坚固且半渗透的水凝胶结构，已用于重要应用，例如梯度产生和研究细胞信号传导。然而，一项挑战仍未解决。当将具有流动组装的去质子化壳聚糖膜（DCM）的聚二甲基硅氧烷（PDMS）微通道用抗粘剂（例如Pluronic F-127）处理以防止生物分子和细胞吸附在PDMS上时，DCM和PDMS之间的相互作用会受到损害，并且DCM容易分层。为了解决这一挑战，微流体中的DCM与戊二醛交联以调节其性能，并研究了戊二醛处理的壳聚糖膜（GTCM）的性能变化。首先，确认了GTCM的耐酸性，其抗静水压力的机械强度显着提高，经过Pluronic处理后，仍可在PDMS上保持完整。其次，使用定量偏振光显微镜（qPLM）研究了DCM和GTCM中的结晶，结果表明GTCM的光学延迟和各向异性较低，这意味着分子排列比DCM中的少。最后，通过FITC标记的葡聚糖转运实验对膜的渗透性进行了测试，结果表明，跨GTCM的转运略高于跨DCM的转运。总体而言，戊二醛交联的壳聚糖膜具有更好的耐酸性，在Pluronic处理下具有更高的强度，并且分子微排列更少，同时保留了半渗透性。
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