Cylindrospermopsin通过Rho / ROCK信号传导途径损害细胞骨架并促进血管内皮细胞凋亡，从而诱导异常血管发育。,Environmental Research

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Cylindrospermopsin通过Rho / ROCK信号传导途径损害细胞骨架并促进血管内皮细胞凋亡，从而诱导异常血管发育。
Environmental Research ( IF 7.7 ) Pub Date : 2020-02-07 , DOI: 10.1016/j.envres.2020.109236
Linping Wang ₁ , Guoliang Chen ₁ , Guosheng Xiao ₂ , Lin Han ₂ , Qilong Wang ₂ , Tingzhang Hu ₁

Affiliation

Cylindrospermopsin（CYN）是水体内广泛分布的蓝细菌毒素，被认为对人类和环境健康构成越来越大的威胁。尽管已经报道了其潜在的毒性，但对其血管系统的作用知之甚少。在这项研究中，我们研究了斑马鱼胚胎和人脐静脉内皮细胞（HUVEC）对CYN的毒性作用及其对血管发育的影响，以及CYN诱导的血管毒性作用的可能机制。CYN暴露引起异常的血管发育，并导致普通心静脉（CCV）增长，其中CCV重塑被延迟，这是由更大的CCV面积和更宽的腹径所反映的。CYN降低HUVEC的生存能力，抑制HUVEC迁移，促进HUVEC凋亡，破坏细胞骨架，并增加细胞内ROS水平。此外，CYN可以促进Bax，Bcl-2和MLC-1的表达，并抑制ITGB1，Rho，ROCK和VIM-1的表达。两者合计，CYN可能通过Rho / ROCK信号通路诱导细胞骨架损伤并促进血管内皮细胞凋亡，从而导致异常的血管发育。目前的结果提供了对CYN毒性在心血管疾病中的机制的潜在见解，并且对于了解CYN对水生生物和人类健康的环境风险是有益的。

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Cylindrospermopsin induces abnormal vascular development through impairing cytoskeleton and promoting vascular endothelial cell apoptosis by the Rho/ROCK signaling pathway.

Cylindrospermopsin (CYN) is a widely distributed cyanobacterial toxin in water bodies and is considered to pose growing threats to human and environmental health. Although its potential toxicity has been reported, its effects on the vascular system are poorly understood. In this study, we examined the toxic effects of CYN on vascular development and the possible mechanism of vascular toxicity induced by CYN using zebrafish embryos and human umbilical vein endothelial cells (HUVECs). CYN exposure induced abnormal vascular development and led to an increase in the growth of common cardinal vein (CCV), in which CCV remodeling was delayed as reflected by the larger CCV area and wider ventral diameter. CYN decreased HUVECs viability, inhibited HUVECs migration, promoted HUVECs apoptosis, destroyed cytoskeleton, and increased intracellular ROS levels. Additionally, CYN could promote the expression of Bax, Bcl-2, and MLC-1 and inhibit the expression of ITGB1, Rho, ROCK, and VIM-1. Taken together, CYN may induce cytoskeleton damage and promote vascular endothelial cell apoptosis by the Rho/ROCK signaling pathway, leading to abnormal vascular development. The current results provide potential insight into the mechanism of CYN toxicity in angiocardiopathy and are beneficial for understanding the environmental risks of CYN for aquatic organisms and human health.

更新日期：2020-02-07

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