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In Situ Fluorescence Imaging of the Levels of Glycosylation and Phosphorylation by a MOF-Based Nanoprobe in Depressed Mice.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-02-06 , DOI: 10.1021/acs.analchem.9b04878 Wei Zhang 1 , Xiaolei Liu 1 , Ping Li 1 , Wen Zhang 1 , Hui Wang 1 , Bo Tang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-02-06 , DOI: 10.1021/acs.analchem.9b04878 Wei Zhang 1 , Xiaolei Liu 1 , Ping Li 1 , Wen Zhang 1 , Hui Wang 1 , Bo Tang 1
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Elucidating the relationship between glycosylation and phosphorylation of protein post-translational modifications is of great significance for understanding most diseases. Mass spectrometry has been widely used in research of protein phosphorylation and glycosylation. However, to realize in situ and dynamic analysis of the levels of phosphorylation and glycosylation in cells and in vivo, mass spectrometry still has certain difficulties. Herein, a nano-MOF-based fluorescent probe with Zr(IV) and boric acid as the active center was designed and prepared. Fluorescence detection and imaging of phosphate is achieved through the specific interaction of Zr(IV) and phosphate. With aim to achieve specific recognition of glycosylation sites, the boronic acid group was modified in the MOF structure, and the fluorescence of the MOFs was regulated by the alizarin red. Thus, the glycosylation sites were recognized by the competition between alizarin red and glycosyl. Finally, the nanoprobe was successfully applied for in situ fluorescence imaging of the levels of glycosylation and phosphorylation in depressed mice.
中文翻译：
通过基于MOF的纳米探针在抑郁小鼠中糖基化和磷酸化水平的原位荧光成像。
阐明糖基化和蛋白质翻译后修饰的磷酸化之间的关系对于理解大多数疾病具有重要意义。质谱已广泛用于蛋白质磷酸化和糖基化的研究。然而，为了实现细胞和体内磷酸化和糖基化水平的原位和动态分析，质谱法仍然存在一定的困难。本文中，设计并制备了以Zr（IV）和硼酸为活性中心的纳米MOF基荧光探针。磷酸盐的荧光检测和成像是通过Zr（IV）与磷酸盐的特定相互作用实现的。为了实现对糖基化位点的特异性识别，在MOF结构中修饰了硼酸基团，MOFs的荧光由茜素红调节。因此，通过茜素红和糖基之间的竞争识别糖基化位点。最后，纳米探针已成功地用于抑郁小鼠糖原化和磷酸化水平的原位荧光成像。
更新日期：2020-02-21
中文翻译：
通过基于MOF的纳米探针在抑郁小鼠中糖基化和磷酸化水平的原位荧光成像。
阐明糖基化和蛋白质翻译后修饰的磷酸化之间的关系对于理解大多数疾病具有重要意义。质谱已广泛用于蛋白质磷酸化和糖基化的研究。然而，为了实现细胞和体内磷酸化和糖基化水平的原位和动态分析，质谱法仍然存在一定的困难。本文中，设计并制备了以Zr（IV）和硼酸为活性中心的纳米MOF基荧光探针。磷酸盐的荧光检测和成像是通过Zr（IV）与磷酸盐的特定相互作用实现的。为了实现对糖基化位点的特异性识别，在MOF结构中修饰了硼酸基团，MOFs的荧光由茜素红调节。因此，通过茜素红和糖基之间的竞争识别糖基化位点。最后，纳米探针已成功地用于抑郁小鼠糖原化和磷酸化水平的原位荧光成像。
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