A series of ten new compounds (7a–j) has been synthesized by absolutely replacing the glutamic acid part of Pemetrexed drug, chemically known as N-{4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl}-l-glutamic acid, with primary, secondary, and aryl amines in high yields using diethylphosphorocyanidate (DEPC) as a peptide coupling agent. All the synthesized compounds are characterized by ¹H and ¹³C NMR, LCMS, and FT-IR spectral techniques. All the synthesized novel non-glutamate 4-(2-(6-amino-4-oxo-4,5-dihydro-1H-pyrrolo[2,3-d]pyrimidin-3-yl)ethyl)benzamide derivatives showed 4- to 7-folds higher antiviral activity than its structurally similar commercial drug Pemetrexed against Newcastle disease virus, an avian paramyxovirus. Among the lot, compounds possessing carboxamide synthesized using five-membered heteroaryl amines (7i and 7j) exhibited the highest antiviral activity.

中文翻译：

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新型4-（2-（6-（4-氨基-4-氧代-4,5-二氢-1H-吡咯并[2,3-d]嘧啶-3-基）乙基）苯甲酰胺衍生物的合成及抗病毒研究

通过绝对替代培美曲塞药物的谷氨酸部分（化学名称为N- {4- [2-（2-（2-氨基-4-氧代-4,7-二氢]）），合成了一系列十个新化合物（7a–j） -3H-吡咯并[2，3-d]嘧啶-5-基）乙基]苯甲酰基} -1-谷氨酸，具有伯胺，仲胺和芳基胺，使用二乙基磷腈基氰酸酯（DEPC）作为肽偶联剂可以高收率。所有合成的化合物的特征在于¹ H和¹³13 C NMR，LCMS和FT-IR光谱技术。所有合成的新型非谷氨酸4-（2-（6-氨基-4-氧代-4,5-二氢-1H-吡咯并[2,3-d]嘧啶-3-基）乙基）苯甲酰胺衍生物均显示4-其抗病毒活性比其结构相似的商业药物Pemetrexed抗禽副粘病毒新城疫病毒高7倍。其中，具有使用五元杂芳基胺（7i和7j）合成的具有羧酰胺的化合物表现出最高的抗病毒活性。