<br>褪黑激素对冷冻保存后牛精子特性和超微结构变化的影响。,Veterinary Medicine and Science

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褪黑激素对冷冻保存后牛精子特性和超微结构变化的影响。
Veterinary Medicine and Science ( IF 1.8 ) Pub Date : 2019-12-02 , DOI: 10.1002/vms3.224
A R ChaithraShree ₁ , Shailesh D Ingole ₂ , Vikas D Dighe ₃ , Anagha S Nagvekar ₂ , Simin V Bharucha ₂ , Nilesh R Dagli ₄ , Prakash M Kekan ₂ , Shambhudeo D Kharde ₂

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精液冷冻保存过程中活性氧（ROS）的产生会改变精子的运动和活动特性，导致人工授精（AI）后受孕率低下或失败。褪黑激素是一种抗氧化剂，能够调节 ROS 的作用，并通过减少冷冻过程中的氧化应激来防止精子产生。将八头健康 HF 公牛的八份精液用 Tris 蛋黄甘油增量剂稀释后分成五等份。计算机辅助精液分析仪 (CASA) 结果显示，解冻后对照样品和褪黑激素处理样品之间没有显着差异；然而，关于 VAP、VSL 的精子速度在 0.25 mM MLT 处理的精液中显示出最高的增加，其次是 0.1 mM MLT 处理的精液，但 VCL 除外，其速度随着褪黑激素浓度的增加而增加。 BCF、STR 和 LIN 的精子活力在 0.25 mM MLT 处理的精液中增加最高，其次是 0.1 mM MLT 处理的精液，但 ALH 除外，其中活力随着褪黑激素浓度的增加而增加。电子显微图像显示，与对照组相比，添加 0.1 mM 褪黑素可以保护质膜和顶体区域，并保持冷冻精子超微结构的完整性，而用 0.2 和 0.25 mM 褪黑素处理的精子的电子显微图像显示损伤最高质膜和顶体膜。由此得出结论，在精子稀释剂中添加褪黑激素可以改善冻存公牛精子的解冻后质量。

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Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation.

The production of reactive oxygen species (ROS) during cryopreservation of semen alters the sperm motion and mobility characteristics, resulting in poor or failure of conception rate after artificial insemination (AI). Melatonin an antioxidant is able to modulate the effect of ROS and prevents spermatozoa by reducing the oxidative stress during freezing process. Eight ejaculates from eight healthy HF bulls diluted with Tris egg yolk glycerol extender were divided into five equal aliquots. The Computer Assisted Semen Analyser (CASA) results showed no significant difference between the control—post‐ thaw samples and melatonin‐treated samples; however, the velocity of spermatozoa with regard to the VAP, VSL showed highest increase in the 0.25 mM MLT‐treated semen followed by 0.1 mM MLT treated semen except for VCL where velocity increased with increase in the concentration of melatonin. The vigour of spermatozoa regard to BCF, STR and LIN recorded highest increase in the 0.25 mM MLT treated semen followed by 0.1 mM MLT‐treated semen except for the ALH where vigour increased with increase in the concentration of melatonin. The electron micrography images illustrated that the addition of 0.1 mM melatonin protected the plasma membrane and acrosome region and maintained the ultrastructure integrity of the cryopreserved spermatozoa when compared to control group, whereas the electron micrography of spermatozoa treated with 0.2 and 0.25 mM melatonin illustrated highest damage to the plasma and acrosome membrane. Thus concluding that inclusion of melatonin to sperm extender can improve the post‐thaw quality of cryopreserved bull spermatozoa.

更新日期：2019-12-02

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