Cancer remains one of the leading causes of death worldwide. Introduction of natural compounds with anticancer properties can be an effective step in the prevention and treatment of cancer. Antimicrobial peptides (AMPs) are short peptides whose anticancer activities have been proved previously. In the present study, Enterocin-P (EntP) as a bacteriocin of E. faecium was expressed in HEK expression system by pcDNA3.1 + vector. The recombinant peptide was purified from culture medium using Ni²⁺ affinity chromatography with an average yield of 0.6 mg/ml. The cytotoxic activity of the recombinant peptide was determined toward some cancer cell lines including: SW1353, HUH7, Huh-7.5, C26, B16F0 and NIH3T3 as a normal cell line. Our results showed that EntP peptide had selective cytotoxicity activity only against C26 (IC₅₀: 0.32 mg/ml) and SW1353 (IC₅₀: 1.36 mg/ml) as cancer cell and did not show cytotoxic properties against normal cell. In the second phase of our study, to better understand the mechanisms of EntP peptide, we have tried to predict the possible interaction of this peptide to predominantly negative charge molecules in the cell membrane of cancer cells. Our in-silico analysis revealed that EntP peptide has strong tendency to chondroitin sulfate (− 189 ± 6.24 kJ/mol) and heparan sulfate (− 115 ± 5.12 kJ/mol) as two well-known anionic molecules on surface cancer cells.

中文翻译：

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Enterocin-P在HEK平台中的表达：通过分子建模评估其对癌细胞系的细胞毒性作用及其与细胞表面糖胺聚糖相互作用的能力

癌症仍然是全球范围内主要的死亡原因之一。引入具有抗癌特性的天然化合物可以是预防和治疗癌症的有效步骤。抗菌肽（AMPs）是一种短肽，其抗癌活性先前已得到证明。在本研究中，肠杆菌素-P（EntP）作为粪肠球菌的细菌素通过pcDNA3.1 +载体在HEK表达系统中表达。使用Ni ²⁺从培养基中纯化重组肽亲和层析，平均产量为0.6 mg / ml。确定重组肽对某些癌细胞系的细胞毒活性，包括：SW1353，HUH7，Huh-7.5，C26，B16F0和NIH3T3作为正常细胞系。我们的结果表明，EntP肽仅对作为癌细胞的C26（IC ₅₀：0.32 mg / ml）和SW1353（IC ₅₀：1.36 mg / ml）具有选择性的细胞毒性活性，对正常细胞没有细胞毒性。在研究的第二阶段，为了更好地了解EntP肽的机制，我们试图预测该肽与癌细胞细胞膜中主要为负电荷分子的可能相互作用。我们的硅 分析表明，EntP肽有很强的倾向将硫酸软骨素（-189±6.24 kJ / mol）和硫酸乙酰肝素（-115±5.12 kJ / mol）作为表面癌细胞上的两个著名阴离子分子。