Cu(I)-谷胱甘肽复合物：影响其形成和产生活性氧的能力的因素,Transition Metal Chemistry

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Cu(I)-谷胱甘肽复合物：影响其形成和产生活性氧的能力的因素
Transition Metal Chemistry ( IF 1.6 ) Pub Date : 2010-02-04 , DOI: 10.1007/s11243-010-9330-9
Margarita E. Aliaga , Catalina Carrasco-Pozo , Camilo López-Alarcón , Hernán Speisky

Cu2+ 离子和还原型谷胱甘肽 (GSH) 迅速相互作用形成生理上发生的 Cu(I)-[GSH]2 复合物。受最近报道的这种复合物从分子氧产生超氧自由基的能力的推动，本研究解决了 Cu2+ 和 GSH 的浓度、pH 值和温度如何影响 Cu(I)–[GSH]2 复合物的形成及其产生超氧自由基和过氧化氢的能力。以 1:3 的固定摩尔比添加的 Cu2+ 和 GSH 的浓度增加，导致超氧阴离子、过氧化氢和氧化型谷胱甘肽 (GSSG) 的产量成比例增加。发现 GSSG 的形成与 Cu(I)-[GSH]2 的形成密切相关。pH 值的生物学相关变化（例如，从 6.8 到 7。7) 和温度（从 22 到 37 °C）不影响 Cu(I)-[GSH]2 的形成，正如通过 GSSG 生产评估的那样。然而，随着pH值的增加，超氧自由基的产生增加。在过氧化氢生产方面观察到相反的效果。新制备的 Cu(I)-[GSH]2 复合物（在其形成后一分钟内测定）产生超氧自由基的能力随着温度的升高而增加。然而，这种能力与温度成反比，在检测超氧化物之前，较早提到的制剂在氧气存在下孵育 30 分钟。在后一种条件下，过氧化氢随时间线性积累，

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The Cu(I)–glutathione complex: factors affecting its formation and capacity to generate reactive oxygen species

Cu2+ ions and reduced glutathione (GSH) swiftly interact to form the physiologically occurring Cu(I)–[GSH]2 complex. Prompted by the recently reported ability of this complex to generate superoxide radicals from molecular oxygen, the present study addressed how the concentration of Cu2+ and GSH, the pH, and the temperature affect the formation of the Cu(I)–[GSH]2 complex and its capacity to generate superoxide radicals and hydrogen peroxide. Increasing concentrations of Cu2+ and GSH, added at a fixed molar ratio of 1:3, led to a proportionally greater production of superoxide anions, hydrogen peroxide, and oxidized glutathione (GSSG). GSSG formation was found to closely reflect the formation of Cu(I)–[GSH]2. Biologically relevant changes in pH (e.g., from 6.8 to 7.7) and temperature (from 22 to 37 °C) did not affect the formation of the Cu(I)–[GSH]2, as assessed by GSSG production. However, production of superoxide radicals increased as the pH values were incremented. An opposite effect was observed regarding hydrogen peroxide production. The ability of a freshly prepared Cu(I)–[GSH]2 complex (assayed within a minute from its formation) to generate superoxide radicals was incremented by as the temperature was increased. Such ability, however, correlated inversely with the temperature when, before assaying for superoxide, the earlier referred preparation was incubated during 30 min in the presence of oxygen. Under the latter condition, hydrogen peroxide linearly accumulated in time, suggesting that an increased autodismutation underlies the apparent time-dependent “aging” of the capacity of the complex to generate superoxide.

更新日期：2010-02-04

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