太空飞行和模拟微重力条件会增加果蝇黑腹果蝇感染模型中粘质沙雷氏菌的毒力。,npj Microgravity

当前位置： X-MOL 学术 › npj Micrograv. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

太空飞行和模拟微重力条件会增加果蝇黑腹果蝇感染模型中粘质沙雷氏菌的毒力。
npj Microgravity ( IF 4.4 ) Pub Date : 2020-02-04 , DOI: 10.1038/s41526-019-0091-2
Rachel Gilbert ₁ , Medaya Torres ₂ , Rachel Clemens ₂ , Shannon Hateley ₃ , Ravikumar Hosamani ₁ , William Wade ₂ , Sharmila Bhattacharya ₄

Affiliation

尽管已经证明宇航员在航天飞行后会遭受免疫失调，但其根本原因仍知之甚少，在复杂环境中研究免疫系统时，要考虑许多变量。此外，越来越多的证据表明，不仅免疫系统发生了变化，而且感染宿主的病原体也受到航天和地面航天条件的显着影响。在这项研究中，我们证明了在国际空间站上生长后，粘质沙雷氏菌（Db11菌株）对果蝇的致死力要比地面对照高，但在细菌培养后，粘质沙雷氏菌的毒力表型并未持续。经过地面。使用旋转壁容器在模拟微重力条件下在地面上生长的细菌中也观察到了高毒力。在野生型苍蝇和那些具有明确免疫途径Imd和Toll突变的苍蝇之间，空载细菌的毒力增加幅度相似，这表明感染后宿主免疫系统的变化可能不是主要的致病因素。有助于增加被空间传播细菌感染的地面蝇类的易感性。细菌的特征表明，在以后的时间点，航天细菌在体外和在宿主体内的生长速度均高于地面对照。这些结果表明，在太空环境中，致病细菌会发生复杂的生理变化，

"点击查看英文标题和摘要"

Spaceflight and simulated microgravity conditions increase virulence of Serratia marcescens in the Drosophila melanogaster infection model.

While it has been shown that astronauts suffer immune disorders after spaceflight, the underlying causes are still poorly understood and there are many variables to consider when investigating the immune system in a complex environment. Additionally, there is growing evidence that suggests that not only is the immune system being altered, but the pathogens that infect the host are significantly influenced by spaceflight and ground-based spaceflight conditions. In this study, we demonstrate that Serratia marcescens (strain Db11) was significantly more lethal to Drosophila melanogaster after growth on the International Space Station than ground-based controls, but the increased virulence phenotype of S. marcescens did not persist after the bacterial cultures were passaged on the ground. Increased virulence was also observed in bacteria that were grown in simulated microgravity conditions on the ground using the rotating wall vessel. Increased virulence of the space-flown bacteria was similar in magnitude between wild-type flies and those that were mutants for the well-characterized immune pathways Imd and Toll, suggesting that changes to the host immune system after infection are likely not a major factor contributing towards increased susceptibility of ground-reared flies infected with space-flown bacteria. Characterization of the bacteria shows that at later timepoints spaceflight bacteria grew at a greater rate than ground controls in vitro, and in the host. These results suggest complex physiological changes occurring in pathogenic bacteria in space environments, and there may be novel mechanisms mediating these physiological effects that need to be characterized.

更新日期：2020-02-04

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南