The present study focuses on the synthesis and evaluation of neomycin-loaded hydrogels as potential substrate for wound healing application. Herein, genipin crosslinked gelatin interpenetrated diosgenin-modified nanocellulose (DGN-NC) hydrogels were synthesized. The hydrogels' chemical structures as well as surface morphology, mechanical property, and thermal behavior were characterized. Swelling analysis and gelation kinetics of the hydrogels were studied and the results obtained showed good swelling capacity as well as high gel yield. In addition, the prepared loaded hydrogels were evaluated for antibacterial activity against human pathogenic E. coli and S. aureus bacteria with inhibition capacity determined in the range of 50–88%. In vitro cytocompatibility and drug release studies were also explored under simulated physiological conditions achieving high cell viability and release percentage >80% and >90% after 24 h, respectively. In effect, the design hydrogels in the present study possess adequate incorporated antibacterial properties with significant potentials towards wound dressing and healing applications.

本研究着重于合成和评估载有新霉素的水凝胶作为伤口愈合应用的潜在底物。本文中，合成了京尼平交联的明胶渗透的薯gen皂苷元修饰的纳米纤维素（DGN-NC）水凝胶。表征了水凝胶的化学结构以及表面形态，机械性能和热行为。研究了水凝胶的溶胀分析和凝胶动力学，所得结果显示出良好的溶胀能力和高凝胶产率。另外，评估了制备的负载水凝胶对人病原性大肠杆菌和金黄色葡萄球菌的抗菌活性。确定抑制能力在50–88％范围内的细菌。在模拟的生理条件下，还进行了体外细胞相容性和药物释放研究，以实现高细胞活力，并在24小时后释放百分比分别> 80％和> 90％。实际上，本研究中设计的水凝胶具有足够的结合抗菌特性，在伤口包扎和愈合应用方面具有巨大潜力。