Liquid crystal (LC), a characteristic substance of biofilms, has been reported to positively affect cell affinity. To better combine and utilize the properties of an LC and the advantages of polyurethane (PU) elastomers, the three-dimensional printing (3DP) molding technology and the simple soaking–swelling blending technology were used to construct PU/LC 3D composite scaffolds, and the compressive strength, porosity, hydrophilicity, and in vitro cell experiments of the scaffolds were initially discussed. The results indicated that the newly developed PU/LC 3D composite scaffolds exhibited an LC state; the addition of an LC did not change the porosity after swelling while maintaining a high porosity; the compressive strength of the composite scaffolds decreased while still maintaining high mechanical properties and enhancing hydrophilicity. At the same time, it could improve the cell affinity on the surface of the material, which was beneficial to increase the cell adhesion rate and cell activity, promote the osteogenic differentiation of human mesenchymal stem cells grown on the materials, and improve the alkaline phosphatase activity, calcium nodules, and the expression of related osteogenic genes and proteins. These results demonstrated potential applications of PU/LC composite scaffolds in repairing or regeneration of bone tissue engineering.

中文翻译：

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液晶态三维复合聚氨酯支架的构建及生物相容性

液晶（LC）是生物膜的特征物质，据报道对细胞亲和力有积极影响。为了更好地结合和利用LC的特性以及聚氨酯（PU）弹性体的优势，三维印刷（3DP）成型技术和简单的浸泡-溶胀共混技术被用于构建PU / LC 3D复合支架，并且首先讨论了支架的抗压强度，孔隙率，亲水性和体外细胞实验。结果表明，新开发的PU / LC 3D复合支架呈现LC状态。添加LC不会使溶胀后的孔隙率保持较高的孔隙率；复合支架的抗压强度下降，同时仍保持较高的机械性能并增强亲水性。同时，可以提高材料表面的细胞亲和力，有利于提高细胞黏附率和细胞活性，促进材料上人间充质干细胞的成骨分化，改善碱性磷酸酶。活性，钙结节以及相关成骨基因和蛋白质的表达。这些结果证明了PU / LC复合支架在修复或再生骨组织工程中的潜在应用。以及相关成骨基因和蛋白质的表达。这些结果证明了PU / LC复合支架在修复或再生骨组织工程中的潜在应用。以及相关成骨基因和蛋白质的表达。这些结果证明了PU / LC复合支架在修复或再生骨组织工程中的潜在应用。