The main aim of this work was to firstly develop a selective oxidation approach for biologically converting 5-hydroxymethylfurfural and furfural into the corresponding furan-based carboxylic acids with recombinant Escherichia coli HMFOMUT. Whole-cells of this recombinant strain harbored good biocatalytic activity in a narrow pH range (pH 6.5-7.0), which had high tolerance toward furfural (up to 50 mM) and 5-hydroxymethylfurfural (up to 150 mM), well-known potential inhibitors against microorganisms. 5-Hydroxymethyl-2-furancarboxylic acid and furoic acid could be obtained at 96.9% and 100% yield from 5-hydroxymethylfurfural (150 mM) and furfural (50 mM) at 30 °C and pH 7.0. The improved substrate tolerance of Escherichia coli HMFOMUT is gaining a great interest to synthesize value-added furan-based carboxylic acids, which has potential industrial applications.

中文翻译：

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改进的生物合成衍生的呋喃中5-羟甲基-2-呋喃甲酸和糠酸的生物合成，对重组大肠杆菌HMFOMUT全细胞具有较高的底物耐受性。

这项工作的主要目的是首先开发一种选择性氧化方法，以利用重组大肠杆菌HMFOMUT将5-羟甲基糠醛和糠醛生物转化为相应的呋喃基羧酸。该重组菌株的全细胞在狭窄的pH范围（pH 6.5-7.0）内具有良好的生物催化活性，对糠醛（最高50 mM）和5-羟甲基糠醛（最高150 mM）具有较高的耐受性，众所周知的潜力微生物抑制剂。在30°C和pH 7.0下，由5-羟甲基糠醛（150 mM）和糠醛（50 mM）可以96.9％和100％的产率获得5-羟甲基-2-呋喃甲酸和糠酸。大肠杆菌HMFOMUT对底物耐受性的提高引起人们对合成增值的呋喃基羧酸的浓厚兴趣，