来自 GABA 编辑的 MRS 在 3 T 下的体内 Glx 和 Glu 测量,NMR in Biomedicine

当前位置： X-MOL 学术 › NMR Biomed. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

来自 GABA 编辑的 MRS 在 3 T 下的体内 Glx 和 Glu 测量
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-01-28 , DOI: 10.1002/nbm.4245
Tiffany Bell _{1,

2} , Elodie S Boudes _{1,

2} , Rachelle S Loo _{1,

2} , Gareth J Barker ₃ , David J Lythgoe ₃ , Richard A E Edden _{4,

5} , R Marc Lebel ₆ , Martin Wilson ₇ , Ashley D Harris _{1,

2}

Affiliation

使用 MRS 对谷氨酸 (Glu) 和 γ-氨基丁酸 (GABA) 进行体内定量通常使用两个单独的序列来实现：Glu 的短回波点分辨光谱 (PRESS) 采集和 Mescher-Garwood PRESS (MEGA-PRESS)收购 GABA。本研究的目的是检查从两个不同的 GABA 编辑的 MEGA-PRESS 采集（GABA 加大分子、GABA+、T _E = 68 ms 和大分子抑制）量化的 Glu 和 Glx（谷氨酸 + 谷氨酰胺的组合信号）的一致性, MMSup, T _E = 80 ms)，Glu 和 Glx 从短回波 PRESS (PRESS-35, T _E= 35 毫秒）采集。^{15 名健康男性志愿者接受了单次扫描，其中使用 3 × 3 × 3 cm 3}体素大小在感觉运动和前扣带回皮层的三个采集（GABA+、MMSup 和 PRESS-35）获取数据. 使用 OFF 子光谱和差异 (DIFF) 光谱从 MEGA-PRESS 数据中量化 Glx 和 Glu。使用相关分析、Bland-Altman 图和组内相关系数评估一致性。从 GABA+ 和 MMSup 采集的 OFF 子光谱量化的 Glx 在两个大脑区域显示与 PRESS-35 的一致性差。在感觉运动皮层中，从 GABA+ 的 OFF 子光谱中量化的 Glu 与 PRESS-35 数据显示出适度的一致性，但这一发现在前扣带皮层中没有被复制。使用任一 MEGA-PRESS 序列的 DIFF 光谱量化的 Glx 和 Glu 与两个脑区的 PRESS-35 数据的一致性较差。总之，从 MEGA-PRESS 数据测量的 Glx 和 Glu 通常与使用 PRESS-35 测量的 Glx 和 Glu 的一致性较差。

"点击查看英文标题和摘要"

In vivo Glx and Glu measurements from GABA‐edited MRS at 3 T

In vivo quantification of glutamate (Glu) and γ‐aminobutyric acid (GABA) using MRS is often achieved using two separate sequences: a short‐echo point resolved spectroscopy (PRESS) acquisition for Glu and a Mescher‐Garwood PRESS (MEGA‐PRESS) acquisition for GABA. The purpose of this study was to examine the agreement of Glu and Glx (the combined signal of glutamate + glutamine) quantified from two different GABA‐edited MEGA‐PRESS acquisitions (GABA plus macromolecules, GABA+, T_E = 68 ms, and macromolecule suppressed, MMSup, T_E = 80 ms) with Glu and Glx quantified from a short‐echo PRESS (PRESS‐35, T_E = 35 ms) acquisition. Fifteen healthy male volunteers underwent a single scan session, in which data were acquired using the three acquisitions (GABA+, MMSup and PRESS‐35) in both the sensorimotor and anterior cingulate cortices using a voxel size of 3 × 3 × 3 cm³. Glx and Glu were quantified from the MEGA‐PRESS data using both the OFF sub‐spectra and the difference (DIFF) spectra. Agreement was assessed using correlation analyses, Bland–Altman plots and intraclass correlation coefficients. Glx quantified from the OFF sub‐spectra from both the GABA+ and MMSup acquisitions showed poor agreement with PRESS‐35 in both brain regions. In the sensorimotor cortex, Glu quantified from the OFF sub‐spectra of GABA+ showed moderate agreement with PRESS‐35 data, but this finding was not replicated in the anterior cingulate cortex. Glx and Glu quantified using the DIFF spectra of either MEGA‐PRESS sequence were in poor agreement with the PRESS‐35 data in both brain regions. In conclusion, Glx and Glu measured from MEGA‐PRESS data generally showed poor agreement with Glx and Glu measured using PRESS‐35.

更新日期：2020-01-28

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南