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Enzymic polypeptide antioxidant activity and inhibitory activity on α-glucosidase and α-amylase from Paeonia ostii cake
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2020-01-29 , DOI: 10.1016/j.indcrop.2020.112158
Huiru Qiao , Xiaojuan Bi , Yuanyuan Zhang , Mengran Liu , Shuchong Zu , Nan Jia , Shougang Jiang , Qi Lu , Yuangang Zu , Yihong Bao

As a residue of oil processing, research about Paeonia ostii cake has not been reported. We used six enzymes to hydrolyze the Paeonia ostii cake and obtained six enzymatic polypeptides. The enzymatic polypeptide yields of the six methods were between 10.0 and 25.0 % and average molecular weight was between 3.0 and 10.0 kDa. The average particle size of six enzymatic polypeptides was less than 1000.0 nm. The enzymatic hydrolysis method had a significant effect on the surface morphology of the enzymatic residues. The enzymatic polypeptide from alkaline protease method had better thermal stability and stronger infrared absorption. The eight essential amino acid contents of six enzymatic polypeptides from pepsin, papain, neutral protease, protemax, trypsase and alkaline protease method were 14.7, 13.3, 14.2, 12.8, 13.8 and 15.0 %, and hydrophobic amino acid contents were 14.7, 14.2, 14.2, 13.9, 13.7 and 15.1 %, respectively. Among the 12 non-essential amino acids, only glutamine and asparagine were absent. In addition, the different amino acid increment in six enzymatic polypeptides was significantly different. Among them, cysteine had the largest increment, followed by glycine and glutamic acid. By 5-axe cobweb chart method, the enzymatic polypeptide from alkaline protease method was proved to have the strongest comprehensive antioxidant activity. The enzymatic polypeptides of alkaline protease and protemax method had stronger inhibition activity of α-glucosidase and α-amylase. In general, the enzymatic polypeptide from alkaline protease method had good antioxidant activity and physiological activity. Our research will provide basic data support for new potential applications of enzymatic polypeptide from peony cake and increase the added value of Paeonia ostii processing enterprises.



中文翻译:

Pa药蛋糕中酶多肽的抗氧化活性及对α-葡萄糖苷酶和α-淀粉酶的抑制活性

作为油脂加工的残余物,尚未有关于Pa药cake蛋糕的研究报道。我们使用了六种酶来水解the并得到六种酶促多肽。六种方法的酶多肽产率在10.0至25.0%之间,平均分子量在3.0至10.0 kDa之间。六个酶促多肽的平均粒径小于1000.0 nm。酶水解方法对酶残基的表面形态有重大影响。碱性蛋白酶法制备的酶多肽具有较好的热稳定性和较强的红外吸收性。胃蛋白酶,木瓜蛋白酶,中性蛋白酶,protemax,胰蛋白酶和碱性蛋白酶法的6种酶促多肽的8种必需氨基酸含量分别为14.7%,13.3%,14.2%,12.8%,13.8%和15.0%,疏水性氨基酸含量分别为14.7%,14.2%,14.2% ,分别为13.9、13.7和15.1%。在12种非必需氨基酸中,仅不存在谷氨酰胺和天冬酰胺。另外,六个酶促多肽中不同的氨基酸增量有显着差异。其中,半胱氨酸的增量最大,其次是甘氨酸和谷氨酸。通过五轴蜘蛛网图法,证明碱性蛋白酶法制备的酶多肽具有最强的综合抗氧化活性。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。六个酶促多肽中不同的氨基酸增量有显着差异。其中,半胱氨酸的增量最大,其次是甘氨酸和谷氨酸。通过五轴蜘蛛网图法,证明碱性蛋白酶法制备的酶多肽具有最强的综合抗氧化活性。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。六个酶促多肽中不同的氨基酸增量有显着差异。其中,半胱氨酸的增量最大,其次是甘氨酸和谷氨酸。通过五轴蜘蛛网图法,证明碱性蛋白酶法制备的酶多肽具有最强的综合抗氧化活性。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。其次是甘氨酸和谷氨酸。通过五轴蜘蛛网图法,证明碱性蛋白酶法制备的酶多肽具有最强的综合抗氧化活性。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。其次是甘氨酸和谷氨酸。通过五轴蜘蛛网图法,证明碱性蛋白酶法制备的酶多肽具有最强的综合抗氧化活性。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。碱性蛋白酶和protemax法的酶促多肽对α-葡萄糖苷酶和α-淀粉酶的抑制活性较强。通常,碱性蛋白酶法得到的酶促多肽具有良好的抗氧化活性和生理活性。我们的研究将为牡丹饼中酶促多肽的新潜在应用提供基础数据支持,并增加牡丹皮酶的附加值。e药加工企业。

更新日期:2020-01-30
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