Although health benefits of the Dietary Approaches to Stop Hypertension (DASH) diet are established, it is not understood which food compounds result in these benefits. We used metabolomics to identify unique compounds from individual foods of a DASH-style diet and determined if these Food-Specific Compounds (FSC) are detectable in urine from participants in a DASH-style dietary study. We also examined relationships between urinary compounds and blood pressure (BP). Nineteen subjects were randomized into 6-week controlled DASH-style diet interventions. Mass spectrometry-based metabolomics was performed on 24-hour urine samples collected before and after each intervention and on 12 representative DASH-style foods. Between 66-969 compounds were catalogued as FSC; for example, 4-hydroxydiphenylamine was found to be unique to apple. Overall, 13-190 of these FSC were detected in urine, demonstrating that these unmetabolized food compounds can be discovered in urine using metabolomics. Although linear mixed effects models showed no FSC from the 12 profiled foods were significantly associated with BP, other endogenous and food-related compounds were associated with BP (N = 16) and changes in BP over time (N = 6). Overall, this proof of principle study demonstrates that metabolomics can be used to catalog FSC, which can be detected in participant urine following a dietary intervention.

中文翻译：

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营养代谢组学揭示了成年人食用DASH式饮食的尿液中的食物特定化合物。

尽管已经建立了饮食控制高血压（DASH）饮食对健康的益处，但尚不清楚哪种食物化合物可带来这些益处。我们使用代谢组学方法从DASH式饮食的单个食物中识别出独特的化合物，并确定这些食品特定化合物（FSC）在DASH式饮食研究的参与者的尿液中是否可检测到。我们还检查了尿液化合物与血压（BP）之间的关系。19名受试者被随机分为6周对照的DASH式饮食干预。基于质谱的代谢组学是在每次干预前后收集的24小时尿液样本以及12种具有代表性的DASH型食物上进行的。66-969种化合物被分类为FSC；例如，发现4-羟基二苯胺是苹果特有的。总体，在尿液中检测到13-190种FSC，这表明可以使用代谢组学在尿液中发现这些未代谢的食物化合物。尽管线性混合效应模型显示12种轮廓食品中没有FSC与BP显着相关，但其他内源性和食品相关化合物与BP相关（N = 16）以及BP随时间的变化（N = 6）。总体而言，这项原理研究证明表明，代谢组学可用于对FSC进行分类，在饮食干预后可从参与者尿液中检测出FSC。其他内源性和食品相关的化合物与BP（N = 16）和BP随时间的变化（N = 6）相关。总体而言，这项原理研究证明表明，代谢组学可用于对FSC进行分类，在饮食干预后可从参与者尿液中检测出FSC。其他内源性和食品相关的化合物与BP（N = 16）和BP随时间的变化（N = 6）相关。总体而言，这项原理研究证明表明，代谢组学可用于对FSC进行分类，在饮食干预后可从参与者尿液中检测出FSC。