多肽通过刺激家蝇幼虫的SOD活性和调节线粒体功能来减轻镉诱导的氧化应激。,Chemosphere

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多肽通过刺激家蝇幼虫的SOD活性和调节线粒体功能来减轻镉诱导的氧化应激。
Chemosphere ( IF 8.1 ) Pub Date : 2020-01-22 , DOI: 10.1016/j.chemosphere.2020.126009
Yuming Zhang ₁ , Yajing Li ₂ , Qin Feng ₂ , Menghua Shao ₂ , Fengyu Yuan ₂ , Fengsong Liu ₁

Affiliation

由于电镀和冶金工业的发展，镉（Cd）是一种广泛的环境污染物。镉可以通过食物链被生物富集，从而导致环境问题扩大，对人类健康构成威胁。虎杖（Polydum cuspidatum）衍生的天然stilbenoid化合物Polydatin（PD）对氧化损伤具有明显的治疗作用。在这项工作中，评估了PD对家蝇（家蝇）幼虫中Cd诱导的氧化损伤的保护作用。将幼虫暴露于Cd和/或PD，然后评估其氧化应激状态，线粒体活性，氧化磷酸化效率和存活率。镉暴露导致丙二醛（MDA）显着增加，家蝇幼虫中的活性氧（ROS）和8-羟基-2-脱氧鸟苷（8-oxoG），导致线粒体功能障碍和存活率下降。有趣的是，PD预处理对暴露于Cd的幼虫具有明显的线粒体保护作用，这可通过降低MDA，ROS和8-oxoG水平以及增加超氧化物歧化酶（SOD），线粒体电子转移链和线粒体膜电位来证明，以及呼吸控制率。这些结果表明，PD可以通过维持氧化还原平衡，刺激SOD活性和调节家蝇幼虫的线粒体活性来减轻Cd诱导的伤害。作为天然的多酚类化学物质，PD可以作为缓解Cd损伤的潜在候选化合物。PD预处理对暴露于Cd的幼虫具有明显的线粒体保护作用，这可通过降低MDA，ROS和8-oxoG水平以及增加超氧化物歧化酶（SOD），线粒体电子转移链和线粒体膜电位来证明。作为呼吸控制率。这些结果表明，PD可以通过维持氧化还原平衡，刺激SOD活性和调节家蝇幼虫的线粒体活性来减轻Cd诱导的伤害。作为天然的多酚类化学物质，PD可以作为缓解Cd损伤的潜在候选化合物。PD预处理对暴露于Cd的幼虫具有明显的线粒体保护作用，这可通过降低MDA，ROS和8-oxoG水平以及增加超氧化物歧化酶（SOD），线粒体电子转移链和线粒体膜电位来证明。作为呼吸控制率。这些结果表明，PD可以通过维持氧化还原平衡，刺激SOD活性和调节家蝇幼虫的线粒体活性来减轻Cd诱导的伤害。作为天然的多酚类化学物质，PD可以作为缓解Cd损伤的潜在候选化合物。和线粒体膜电位，以及呼吸控制率。这些结果表明，PD可以通过维持氧化还原平衡，刺激SOD活性和调节家蝇幼虫的线粒体活性来减轻Cd诱导的伤害。作为天然的多酚类化学物质，PD可以作为缓解Cd损伤的潜在候选化合物。和线粒体膜电位，以及呼吸控制率。这些结果表明，PD可以通过维持氧化还原平衡，刺激SOD活性和调节家蝇幼虫的线粒体活性来减轻Cd诱导的伤害。作为天然的多酚类化学物质，PD可以作为缓解Cd损伤的潜在候选化合物。

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Polydatin attenuates cadmium-induced oxidative stress via stimulating SOD activity and regulating mitochondrial function in Musca domestica larvae.

Cadmium (Cd) is a widespread environment contaminant due to the development of electroplating and metallurgical industry. Cd can be enriched by organisms via food chain, causing the enlarged environmental problems and posing threats to the health of humans. Polydatin (PD), a natural stilbenoid compound derived from Polygonum cuspidatum, shows pronouncedly curative effect on oxidative damage. In this work, the protective effects of PD on oxidative damage induced by Cd in Musca domestica (housefly) larvae were evaluated. The larvae were exposed to Cd and/or PD, subsequently, the oxidative stress status, mitochondria activity, oxidative phosphorylation efficiency, and survival rate were assessed. Cd exposure generated significant increases of malondialdehyde (MDA), reactive oxygen species (ROS) and 8-hydroxy-2-deoxyguanosine (8-oxoG) in the housefly larvae, causing mitochondrial dysfunction and survival rate decline. Interestingly, pretreatment with PD exhibited obviously mitochondrial protective effects in the Cd-exposed larvae, as evidenced by reduced MDA, ROS and 8-oxoG levels, and increased activities of superoxide dismutase (SOD), mitochondrial electron transfer chain, and mitochondrial membrane potential, as well as respiratory control ratio. These results suggested that PD could attenuate Cd-induced damage via maintaining redox balance, stimulating SOD activity, and regulating mitochondria activity in housefly larvae. As a natural polyphenolic chemical, PD can act as a potential candidate compounds to relieve Cd injury.

更新日期：2020-01-22

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