<br>来自大肠杆菌的 YqfB 蛋白：一种对 N4-酰基胞嘧啶衍生物具有活性的非典型酰胺水解酶。,Scientific Reports

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来自大肠杆菌的 YqfB 蛋白：一种对 N4-酰基胞嘧啶衍生物具有活性的非典型酰胺水解酶。
Scientific Reports ( IF 3.8 ) Pub Date : 2020-01-21 , DOI: 10.1038/s41598-020-57664-w
Rūta Stanislauskienė ₁ , Audrius Laurynėnas ₂ , Rasa Rutkienė ₁ , Agota Aučynaitė ₁ , Daiva Tauraitė ₁ , Rita Meškienė ₁ , Nina Urbelienė ₁ , Algirdas Kaupinis ₃ , Mindaugas Valius ₃ , Laura Kaliniene ₁ , Rolandas Meškys ₁

Affiliation

含有人类激活信号协整同源（ASCH）结构域的蛋白质广泛且多样，但目前，这些蛋白质中的绝大多数还没有指定的功能。这项研究表明，之前假设的 103 个氨基酸的大肠杆菌蛋白 YqfB 是一种独特的含有 ASCH 结构域的酰胺水解酶，负责 N4-乙酰胞苷 (ac4C) 的分解代谢。 YqfB 有几个有趣且独特的特征：i) 它是迄今为止描述的最小的单体酰胺水解酶，ii) 它对结构不同的 N4-酰化胞嘧啶/胞苷具有活性，iii) 它对这些底物具有高度特异性 (kcat/Km)高达 2.8 × 106 M-1 s-1)。此外，我们的结果表明，YqfB 含有独特的 Thr-Lys-Glu 催化三联体，Arg 充当氧阴离子孔。缺乏 yqfB 基因的突变体保留了以 N4-乙酰胞嘧啶作为尿嘧啶来源生长的能力，尽管很差，这表明大肠杆菌中存在利用该化合物的替代途径。总体而言，YqfB 水解各种 N4 酰化胞嘧啶和胞苷的能力不仅揭示了 ac4C 在细菌中如何分解代谢的长期谜团，而且还扩展了我们对酰胺水解酶活性位点内结构多样性的了解。

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YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N4-acylcytosine derivatives.

Human activating signal cointegrator homology (ASCH) domain-containing proteins are widespread and diverse but, at present, the vast majority of those proteins have no function assigned to them. This study demonstrates that the 103-amino acid Escherichia coli protein YqfB, previously identified as hypothetical, is a unique ASCH domain-containing amidohydrolase responsible for the catabolism of N4-acetylcytidine (ac4C). YqfB has several interesting and unique features: i) it is the smallest monomeric amidohydrolase described to date, ii) it is active towards structurally different N4-acylated cytosines/cytidines, and iii) it has a high specificity for these substrates (kcat/Km up to 2.8 × 106 M-1 s-1). Moreover, our results suggest that YqfB contains a unique Thr-Lys-Glu catalytic triad, and Arg acting as an oxyanion hole. The mutant lacking the yqfB gene retains the ability to grow, albeit poorly, on N4-acetylcytosine as a source of uracil, suggesting that an alternative route for the utilization of this compound exists in E. coli. Overall, YqfB ability to hydrolyse various N4-acylated cytosines and cytidines not only sheds light on the long-standing mystery of how ac4C is catabolized in bacteria, but also expands our knowledge of the structural diversity within the active sites of amidohydrolases.

更新日期：2020-01-22

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