Bioinspired, Artificial, Small-Diameter Vascular Grafts with Selective and Rapid Endothelialization Based on an Amniotic Membrane-Derived Hydrogel,ACS Biomaterials Science & Engineering

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Bioinspired, Artificial, Small-Diameter Vascular Grafts with Selective and Rapid Endothelialization Based on an Amniotic Membrane-Derived Hydrogel
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-02-04 , DOI: 10.1021/acsbiomaterials.9b01493
Xu Peng _{1,

2} , Xu Wang _{1,

3} , Can Cheng ₁ , Xiong Zhou ₁ , Zhipeng Gu ₁ , Li Li ₄ , Jun Liu ₅ , Xixun Yu ₁

Affiliation

Clinical application of the amniotic membrane (AM) in vascular reconstruction was limited by poor processability, rapid biodegradation, and insufficient hemocompatibility. In this work, decellularized AM was digested to a thermosensitive hydrogel and densely cross-linked in the nanoscale as “enhanced” collagenous fibers. Via N-(3-dimehylaminopropyl)-N′-ethylcarbodiimide and N-hydroxysuccinimide (EDC/NHS) catalysis, REDV was further grafted to simulate anticoagulant substances on naturally derived blood vessels. This modification approach endowed AM with rapid endothelialization and rare vascular restenosis. Through adjusting the fixation condition, the pore size and mechanical stability of the fiber network were approximate to those of natural tissues and precisely designed to fit for cell adhesion. AM was synchronously fixed by alginate dialdehyde (ADA) and EDC/NHS, forming a “double-cross-linked” stable structure with significantly improved mechanical strength and resistance against enzymic degradation. The hemolytic and platelet adhesion test indicated that ADA/REDV-AM could inhibit hemolysis and coagulation. It also exhibited excellent cytocompatibility. It selectively accelerated adsorption and migration of endothelial cells (ECs) while impeding adhesion and proliferation of smooth muscle cells (SMCs). It maintained EC superiority in competitive growth and avoided thrombosis in vivo. Furthermore, its property of promoting reconstruction and repair of blood vessels was proved in an animal experiment. Overall, the present study demonstrates that ADA/REDV-AM has potential application as a small-diameter artificial vascular intima with rapid endothelialization and reduced SMC/platelet adhesion.

中文翻译：

基于羊膜水凝胶的具有选择性和快速内皮化的生物启发性，人工，小直径血管移植物。

羊膜（AM）在血管重建中的临床应用受到可加工性差，生物降解迅速和血液相容性不足的限制。在这项工作中，脱细胞的AM被消化成热敏性水凝胶，并在纳米级密集地交联，成为“增强型”胶原纤维。通过N-（3-二乙氨基氨基丙基）-N'-乙基碳二亚胺和N-羟基琥珀酰亚胺（EDC / NHS）催化，将REDV进一步嫁接以模拟天然来源血管上的抗凝物质。这种修饰方法使AM具有快速的内皮化和罕见的血管再狭窄。通过调整固定条件，纤维网络的孔径和机械稳定性与天然组织的孔径和机械稳定性近似，并经过精确设计以适合细胞粘附。通过藻酸盐二醛（ADA）和EDC / NHS同步固定AM，形成“双交联”的稳定结构，机械强度和抗酶降解性显着提高。溶血和血小板粘附试验表明，ADA / REDV-AM可以抑制溶血和凝血。它也表现出优异的细胞相容性。它选择性地加速了内皮细胞（ECs）的吸附和迁移，同时阻碍了平滑肌细胞（SMCs）的粘附和增殖。它在竞争性增长中保持EC优势，并避免体内血栓形成。此外，在动物实验中证明了其具有促进血管重建和修复的特性。总体而言，本研究表明，ADA / REDV-AM具有作为小直径人造血管内膜的潜在应用，具有快速内皮化和减少SMC /血小板粘附的作用。

更新日期：2020-02-04

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