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Self-Polymerized Dopamine-Decorated Au NPs and Coordinated with Fe-MOF as a Dual Binding Sites and Dual Signal-Amplifying Electrochemical Aptasensor for the Detection of CEA.
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-01-24 , DOI: 10.1021/acsami.9b19161 Jifan Li 1, 2 , Lei Liu 2 , Yongjian Ai 1 , Yang Liu 1 , Hongbin Sun 2 , Qionglin Liang 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-01-24 , DOI: 10.1021/acsami.9b19161 Jifan Li 1, 2 , Lei Liu 2 , Yongjian Ai 1 , Yang Liu 1 , Hongbin Sun 2 , Qionglin Liang 1
Affiliation
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Fabrication of functional electrochemical biosensor is a hot topic; however, precise and sensitive cancer detection in early clinical diagnosis is still a great challenge. Continuous efforts have been devoted to explore functional materials for this issue. In this work, we developed a dual binding sites and dual signal-amplifying electrochemical aptasensor of self-polymerized dopamine-decorated Au and coordinated with Fe-MOF (Au@PDA@Fe-MOF) for the detection of carcinoembryonic antigen (CEA). Remarkably, Au@PDA@Fe-MOF features high sensitivity, multiple active sites, good biocompatibility, and excellent selectivity, which is attributed to abundant -COOH in porous Fe-MOF and unsaturated Fe3+ sites on the surface of Fe-MOF as the active binding sites grafting more NH2-functionalized CEA-specific aptamer and redox PDA and Fe-MOF accelerating the movement of electrons for dual signal amplifying. Meanwhile, the electrochemical aptasensor shows favorable repeatability with 1.82% relative standard deviation (RSD) under five independent aptasensors and strong stability with only 3.3% degradation after 12 days of storage. In addition, the aptasensor has wide CEA detection range from 1 fg mL-1 to 1 μg mL-1 with a low detection limit of 0.33 fg mL-1 (S/N = 3). Furthermore, the aptasensor is feasible for accurate and quantitative detection of CEA in serum samples with RSD below 2.32%. The satisfying results demonstrate promising applications of the CEA aptasensor in practical sample analysis and lay an important foundation for other biomarker detection in early clinical diagnosis.
中文翻译：
自聚合多巴胺修饰的金纳米颗粒，并与铁-MOF配合作为双结合位点和双信号放大电化学传感器，用于检测CEA。
功能性电化学生物传感器的制备是一个热门话题。然而，在早期临床诊断中精确而敏感的癌症检测仍然是一个巨大的挑战。为了解决该问题，已经进行了持续的努力来探索功能材料。在这项工作中，我们开发了自聚合的多巴胺修饰的Au的双结合位点和双信号放大电化学适体传感器，并与Fe-MOF（Au @ PDA @ Fe-MOF）配合用于检测癌胚抗原（CEA）。值得注意的是，Au @ PDA @ Fe-MOF具有高灵敏度，多个活性位点，良好的生物相容性和出色的选择性，这归因于多孔Fe-MOF中大量的-COOH和Fe-MOF表面上的不饱和Fe3 +位点，因为它们的活性结合位点接枝了更多的NH2官能化CEA特异性适体和氧化还原PDA，并且Fe-MOF加速了双信号放大。同时，电化学适体在五个独立的适体下显示出良好的可重复性，相对标准偏差（RSD）为1.82％，并且在储存12天后具有仅3.3％降解的强稳定性。此外，适体传感器的CEA检测范围很广，从1 fg mL-1到1μgmL-1，检测限很低，为0.33 fg mL-1（S / N = 3）。此外，适体传感器对于RSD低于2.32％的血清样品中CEA的准确和定量检测是可行的。
更新日期：2020-01-26
中文翻译：
自聚合多巴胺修饰的金纳米颗粒，并与铁-MOF配合作为双结合位点和双信号放大电化学传感器，用于检测CEA。
功能性电化学生物传感器的制备是一个热门话题。然而，在早期临床诊断中精确而敏感的癌症检测仍然是一个巨大的挑战。为了解决该问题，已经进行了持续的努力来探索功能材料。在这项工作中，我们开发了自聚合的多巴胺修饰的Au的双结合位点和双信号放大电化学适体传感器，并与Fe-MOF（Au @ PDA @ Fe-MOF）配合用于检测癌胚抗原（CEA）。值得注意的是，Au @ PDA @ Fe-MOF具有高灵敏度，多个活性位点，良好的生物相容性和出色的选择性，这归因于多孔Fe-MOF中大量的-COOH和Fe-MOF表面上的不饱和Fe3 +位点，因为它们的活性结合位点接枝了更多的NH2官能化CEA特异性适体和氧化还原PDA，并且Fe-MOF加速了双信号放大。同时，电化学适体在五个独立的适体下显示出良好的可重复性，相对标准偏差（RSD）为1.82％，并且在储存12天后具有仅3.3％降解的强稳定性。此外，适体传感器的CEA检测范围很广，从1 fg mL-1到1μgmL-1，检测限很低，为0.33 fg mL-1（S / N = 3）。此外，适体传感器对于RSD低于2.32％的血清样品中CEA的准确和定量检测是可行的。
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