Three Newly Isolated Calcium-Chelating Peptides from Tilapia Bone Collagen Hydrolysate Enhance Calcium Absorption Activity in Intestinal Caco-2 Cells.,Journal of Agricultural and Food Chemistry

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Three Newly Isolated Calcium-Chelating Peptides from Tilapia Bone Collagen Hydrolysate Enhance Calcium Absorption Activity in Intestinal Caco-2 Cells.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-21 , DOI: 10.1021/acs.jafc.9b07602
Wanwen Liao _{1,

2,

3} , Hui Chen ₁ , Wengang Jin ₄ , Zhennai Yang ₃ , Yong Cao ₁ , Jianyin Miao _{1,

2,

3}

Affiliation

In this study, we used reversed-phase high performance liquid chromatography (LC) to isolate three novel peptides with calcium-chelating capacity from tilapia bone collagen hydrolysate. Using LC-tandem mass spectrometry, we determined the amino acid sequences to be GPAGPHGPVG, FDHIVY, and YQEPVIAPKL. We then synthesized the three peptides and verified their calcium-chelating activity. Results showed that the calcium-chelating activity of GPAGPHGPVG, FDHIVY, and YQEPVIAPKL reached 18.80 ± 0.49, 35.73 ± 0.74, and 28.4 ± 0.94 mg/g, respectively. We next investigated how each peptide enhanced intestinal calcium absorption using Caco-2 cell monolayers. Compared with the control group, GPAGPHGPVG, FDHIVY, and YQEPVIAPKL potently enhanced calcium transport within 30 min by 89 ± 9, 202 ± 12, and 130 ± 7%, respectively. Results suggest that these peptides isolated from tilapia bone hydrolysate can be used as dietary supplements to increase calcium absorption.

中文翻译：

罗非鱼骨胶原水解产物中的三种新分离的钙螯合肽增强了肠道Caco-2细胞的钙吸收活性。

在这项研究中，我们使用反相高效液相色谱（LC）从罗非鱼骨胶原水解物中分离出三种具有钙螯合能力的新型肽。使用LC串联质谱，我们确定了氨基酸序列为GPAGPHGPVG，FDHIVY和YQEPVIAPKL。然后，我们合成了这三种肽并验证了它们的钙螯合活性。结果表明，GPAGPHGPVG，FDHIVY和YQEPVIAPKL的钙螯合活性分别达到18.80±0.49、35.73±0.74和28.4±0.94 mg / g。接下来，我们研究了每种肽如何利用Caco-2细胞单层膜增强肠道钙的吸收。与对照组相比，GPAGPHGPVG，FDHIVY和YQEPVIAPKL在30分钟内有效地增强了钙的转运，分别为89±9、202±12和130±7％。

更新日期：2020-01-22

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