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Preparation, Characterization, and Antimicrobial Activity of Cubosome Encapsulated Metal Nanocrystals.
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-01-22 , DOI: 10.1021/acsami.9b21783 Thomas G Meikle 1 , Brendan P Dyett 1 , Jamie B Strachan 1 , Jacinta White 2 , Calum J Drummond 1 , Charlotte E Conn 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-01-22 , DOI: 10.1021/acsami.9b21783 Thomas G Meikle 1 , Brendan P Dyett 1 , Jamie B Strachan 1 , Jacinta White 2 , Calum J Drummond 1 , Charlotte E Conn 1
Affiliation
Herein, we demonstrate a method for the functionalization of cubic phase lipid nanoparticles (cubosomes) with a series of magnetite (Fe3O4), copper oxide (Cu2O), and silver (Ag) nanocrystals, with prospective applications across a wide range of fields, including antimicrobial treatments. The resulting cubosomes are characterized using small-angle X-ray scattering and dynamic light scattering, demonstrating the retention of a typical cubic phase structure and particle size following nanocrystal encapsulation at concentrations up to 20% w/w. Cryogenic transmission electron microscopy reveals significant loading and association of each nanocrystal type with both monoolein- and phytantriol-based cubosomes. The antibiotic potential of these hybrid nanoparticles is demonstrated for the first time; cubosomes with embedded silver nanocrystals display a high level of antimicrobial activity against both Gram-positive and Gram-negative bacteria, with observed minimum inhibitory concentration values ranging from 15.6-250 μg/mL. Lastly, total internal reflection fluorescence microscopy is used to visualize cubosome-bacteria interactions, suggesting the involvement of particle interactions as a delivery mechanism.
中文翻译:
脂质体包裹的金属纳米晶体的制备,表征和抗菌活性。
在本文中,我们演示了利用一系列磁铁矿(Fe3O4),氧化铜(Cu2O)和银(Ag)纳米晶体对立方相脂质纳米颗粒(cubosomes)进行功能化的方法,并有望在广泛的领域中应用,包括抗菌治疗。使用小角度X射线散射和动态光散射对所得的立方体进行表征,证明了纳米晶封装后浓度高达20%w / w的典型立方相结构和粒径的保留。低温透射电子显微镜揭示了每种纳米晶类型与单油精和植物三醇的立方脂质体的显着负载和缔合。首次证明了这些杂化纳米颗粒的抗生素潜力。嵌入银纳米晶体的立方微粒对革兰氏阳性和革兰氏阴性细菌均表现出高水平的抗菌活性,观察到的最小抑菌浓度范围为15.6-250μg/ mL。最后,使用全内反射荧光显微镜观察立方核糖体与细菌的相互作用,这表明颗粒相互作用是一种传递机制。
更新日期:2020-01-23
中文翻译:
脂质体包裹的金属纳米晶体的制备,表征和抗菌活性。
在本文中,我们演示了利用一系列磁铁矿(Fe3O4),氧化铜(Cu2O)和银(Ag)纳米晶体对立方相脂质纳米颗粒(cubosomes)进行功能化的方法,并有望在广泛的领域中应用,包括抗菌治疗。使用小角度X射线散射和动态光散射对所得的立方体进行表征,证明了纳米晶封装后浓度高达20%w / w的典型立方相结构和粒径的保留。低温透射电子显微镜揭示了每种纳米晶类型与单油精和植物三醇的立方脂质体的显着负载和缔合。首次证明了这些杂化纳米颗粒的抗生素潜力。嵌入银纳米晶体的立方微粒对革兰氏阳性和革兰氏阴性细菌均表现出高水平的抗菌活性,观察到的最小抑菌浓度范围为15.6-250μg/ mL。最后,使用全内反射荧光显微镜观察立方核糖体与细菌的相互作用,这表明颗粒相互作用是一种传递机制。