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Continuous Surveillance of Bioaerosols On-Site Using an Automated Bioaerosol-Monitoring System.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-01-08 , DOI: 10.1021/acssensors.9b02001 Yu Sung Cho 1, 2 , Hye Ri Kim 1, 3 , Hyun Sik Ko 1, 2 , Sang Bin Jeong 1, 2 , Byoung Chan Kim 1, 3 , Jae Hee Jung 1, 2, 3
ACS Sensors ( IF 8.2 ) Pub Date : 2020-01-08 , DOI: 10.1021/acssensors.9b02001 Yu Sung Cho 1, 2 , Hye Ri Kim 1, 3 , Hyun Sik Ko 1, 2 , Sang Bin Jeong 1, 2 , Byoung Chan Kim 1, 3 , Jae Hee Jung 1, 2, 3
Affiliation
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Real-time on-site monitoring of bioaerosols in an air environment is important for preventing various adverse health effects including respiratory diseases and allergies caused by bioaerosols. Here, we report the development of an on-site automated bioaerosol-monitoring system (ABMS) using integrated units including a wet-cyclone bioaerosol sampler, a thermal-lysis unit for extracting adenosine triphosphate (ATP), an ATP-detection unit based on the immobilization of luciferase/luciferin for bioluminescence reactions, and a photomultiplier tube-based detector. The performance of the bioaerosol detection system was verified using Escherichia coli (E. coli) as a model source. Each unit was optimized to process ∼9.6 × 105 times the concentrated ratio of collected bioaerosol samples, using a 3 min lysis time to extract ATP, and has a detection limit of ∼375 colony-forming units (CFUs)/mL with more than 30 days of stability for the immobilized-luciferase/luciferin detection unit supported by a glass-fiber conjugation pad. After the integration of all units, the ABMS achieved E. coli bioaerosol monitoring with continuous detection at 5 min intervals and a minimum detection limit of ∼130 CFU/mair3. Furthermore, the rapid responsivity and stable operation performance of the ABMS under test-bed conditions and during a field test demonstrated that the ABMS is capable of continuously monitoring bioaerosols in real-time with high sensitivity. The monitoring system developed here with immobilization strategies for bioluminescence reactions triggered by ATP extracted from collected bioaerosol samples using a simple heat-lysis method may help establish sustainable platforms to obtain stable signals for the real-time detection of bioaerosols on-site.
中文翻译：
使用自动化的生物气溶胶监测系统对现场生物气溶胶进行连续监测。
空气环境中生物气溶胶的实时现场监测对于防止各种不良健康影响（包括呼吸系统疾病和由生物气溶胶引起的过敏）非常重要。在这里，我们报告使用集成单元的现场自动化生物气溶胶监测系统（ABMS）的开发，该集成单元包括湿旋风生物气溶胶采样器，用于提取三磷酸腺苷（ATP）的热裂解单元，基于ATP的检测单元固定萤光素酶/萤光素用于生物发光反应，以及基于光电倍增管的检测器。使用大肠杆菌（E. coli）作为模型来源验证了生物气溶胶检测系统的性能。使用3分钟的裂解时间提取ATP，对每个单元进行优化，使其处理量约为收集的生物气溶胶样品浓缩比的9.6×105倍。检测限为〜375个菌落形成单位（CFU）/ mL，对于由玻璃纤维接合垫支撑的固定化萤光素酶/萤光素检测单元，其稳定性超过30天。所有单元整合后，ABMS以5分钟的间隔连续检测且最低检出限为〜130 CFU / mair3，实现了大肠杆菌生物气溶胶监测。此外，ABMS在试验台条件下和现场测试期间的快速响应能力和稳定的运行性能证明，ABMS能够以高灵敏度实时连续监测生物气溶胶。
更新日期：2020-01-22
中文翻译：
使用自动化的生物气溶胶监测系统对现场生物气溶胶进行连续监测。
空气环境中生物气溶胶的实时现场监测对于防止各种不良健康影响（包括呼吸系统疾病和由生物气溶胶引起的过敏）非常重要。在这里，我们报告使用集成单元的现场自动化生物气溶胶监测系统（ABMS）的开发，该集成单元包括湿旋风生物气溶胶采样器，用于提取三磷酸腺苷（ATP）的热裂解单元，基于ATP的检测单元固定萤光素酶/萤光素用于生物发光反应，以及基于光电倍增管的检测器。使用大肠杆菌（E. coli）作为模型来源验证了生物气溶胶检测系统的性能。使用3分钟的裂解时间提取ATP，对每个单元进行优化，使其处理量约为收集的生物气溶胶样品浓缩比的9.6×105倍。检测限为〜375个菌落形成单位（CFU）/ mL，对于由玻璃纤维接合垫支撑的固定化萤光素酶/萤光素检测单元，其稳定性超过30天。所有单元整合后，ABMS以5分钟的间隔连续检测且最低检出限为〜130 CFU / mair3，实现了大肠杆菌生物气溶胶监测。此外，ABMS在试验台条件下和现场测试期间的快速响应能力和稳定的运行性能证明，ABMS能够以高灵敏度实时连续监测生物气溶胶。
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