Structural Changes and Antibacterial Activity of Epsilon-poly-l-lysine in Response to pH and Phase Transition and Their Mechanisms.,Journal of Agricultural and Food Chemistry

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Structural Changes and Antibacterial Activity of Epsilon-poly-l-lysine in Response to pH and Phase Transition and Their Mechanisms.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-15 , DOI: 10.1021/acs.jafc.9b07524
Jia-Ning Liu _{1,

2} , Sen-Lin Chang _{1,

2} , Peng-Wei Xu _{1,

2} , Ming-Hui Tan _{1,

2} , Bing Zhao _{1,

2} , Xiao-Dong Wang _{1,

2} , Qing-Sheng Zhao _{1,

2}

Affiliation

ε-Poly-l-lysine (ε-PL) consists of 25-35 lysine residues which are linked by an isopeptide bond formed by dehydration condensation of α-carboxyl and ε-amino groups and has good antibacterial activity and broad-spectrum inhibition range. However, there is no clear conclusion about the structure and antibacterial mechanism of ε-PL in aqueous solution. Herein, a high purity of ε-PL was prepared using Amberlite IRC-50 ion-exchange resin. Membrane filtration and dynamic light scattering were used to study the variations of ε-PL aggregation in aqueous solution with pH value. The conformational changes and antibacterial activities of ε-PL and carbamoylated ε-PL in different water environments were studied with circular dichroism (CD) and inhibition zone. The structural changes during the spray-drying process were determined by Fourier transform infrared spectroscopy. The results indicated that the side chain amino charge played a decisive role in the ε-PL conformation and aggregation. ε-PL exhibited the properties of a β-sheet during spray drying from acidic liquids to solids. The cation enhanced the antibacterial activity of ε-PL but did not play a key role. Instead, the backbone of ε-PL might determine the mechanism of ε-PL antibacterial.

中文翻译：

Epsilon-poly-l-赖氨酸的结构变化和抗菌活性对pH和相变的响应及其机理。

ε-聚赖氨酸（ε-PL）由25-35个赖氨酸残基组成，它们通过α-羧基和ε-氨基的脱水缩合形成的异肽键相连，具有良好的抗菌活性和广谱抑制范围。然而，关于水溶液中ε-PL的结构和抗菌机理尚无明确结论。在此，使用Amberlite IRC-50离子交换树脂制备了高纯度的ε-PL。通过膜过滤和动态光散射研究水溶液中ε-PL聚集随pH值的变化。利用圆二色性（CD）和抑制区，研究了不同水环境下ε-PL和氨基甲酸酯化的ε-PL的构象变化和抗菌活性。喷雾干燥过程中的结构变化通过傅立叶变换红外光谱法确定。结果表明，侧链氨基电荷在ε-PL构象和聚集中起决定性作用。从酸性液体到固体的喷雾干燥过程中，ε-PL表现出β-折叠的特性。阳离子增强了ε-PL的抗菌活性，但没有发挥关键作用。相反，ε-PL的骨架可能决定了ε-PL抗菌的机制。

更新日期：2020-01-16

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