The process of modern cardiovascular device fabrication should always be associated with an investigation of how surface properties modulate its hemocompatibility through plasma protein adsorption as well as blood morphotic element activation and adhesion. In this work, a package of novel assays was used to correlate the physicochemical properties of thin ceramic coatings with hemocompatibility under dynamic conditions. Different variants of carbon-based films were prepared on polymer substrates using the magnetron sputtering method. The microstructural, mechanical, and surface physicochemical tests were performed to characterize the coatings, followed by investigation of whole human blood quality changes under blood flow conditions using the “Impact R” test, tubes’ tester, and radial flow chamber assay. The applied methodology allowed us to determine that aggregate formation on hydrophobic and hydrophilic carbon-based coatings may follow one of the two different mechanisms dependent on the type and conformational changes of adsorbed blood plasma proteins.

中文翻译：

---

体外模拟血流条件下薄陶瓷涂层表面血小板的滚动或两阶段聚集

现代心血管设备的制造过程应始终与对表面特性如何通过血浆蛋白吸附以及血液形态元素激活和粘附来调节其血液相容性的研究相关联。在这项工作中，使用了一套新颖的测定方法来将薄陶瓷涂层的物理化学性质与动态条件下的血液相容性联系起来。使用磁控溅射方法在聚合物基底上制备了碳基薄膜的不同变体。进行了微观结构，机械和表面物理化学测试以表征涂层，然后使用“ Impact R”测试，试管测试仪和径向流室测定法研究了在血流条件下全人类血液质量的变化。