一种收集浓缩的富含血小板的纤维蛋白（C-PRF）的新方法，血小板和白血球的产量提高了10倍。,Clinical Oral Investigations

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一种收集浓缩的富含血小板的纤维蛋白（C-PRF）的新方法，血小板和白血球的产量提高了10倍。
Clinical Oral Investigations ( IF 3.1 ) Pub Date : 2019-12-02 , DOI: 10.1007/s00784-019-03147-w
Richard J Miron _{1,

2} , Jihua Chai ₁ , Peng Zhang ₁ , Yuqing Li ₁ , Yunxiao Wang ₁ , Carlos Fernando de Almeida Barros Mourão ₃ , Anton Sculean ₂ , Masako Fujioka Kobayashi ₄ , Yufeng Zhang _{1,

5}

Affiliation

背景和目的液体富血小板纤维蛋白（PRF；通常称为可注射的PRF）已被用作能够刺激组织再生的PRF的可注射制剂。我们的研究小组最近发现，遵循标准的L-PRF协议（2700 RPM，持续12分钟），直接在红细胞层上方的血沉棕黄层中观察到血小板和白细胞的大量增加。这项研究的目的是开发一种新的收获技术，以直接从该血沉棕黄层中分离出液体PRF，并将该技术与标准i-PRF进行比较。材料与方法使用标准的高g力L-PRF和低g力i-PRF方案分离血层。在红血球层上方，连续收获100μL血浆层（共12层；即1.2 mL，代表总的i-PRF体积），从红细胞层收获3层（3×100μL）以量化血细胞。然后将每一层送去进行全血细胞计数（CBC）分析，并对细胞数进行定量，包括红细胞，白细胞，嗜中性粒细胞，淋巴细胞，单核细胞和血小板。根据L-PRF协议直接从血沉棕黄层中收集的液体PRF称为浓缩PRF（C-PRF）。结果与全血中的基线浓度相比，i-PRF方案通常使血小板从1-mL到1.2mL血浆层增加2到3倍，使白细胞浓度增加1.5倍。虽然在L-PRF的第一个4 mL层中几乎没有发现细胞，直接在血沉棕黄层中发现大量血小板和白细胞积聚，表明该0.3-0.5-mL层中的细胞浓度极高（增加了20倍）。因此，我们建议在液体细胞C-PRF的正上方的红细胞小体层上方收获此0.3-0.5 mL的层。通常，i-PRF可使血小板数量增加约250％，而通过收获0.3-0.5 mL C-PRF可以轻松实现血小板数量增加1200-700％（总血小板浓度> 2000- 3000×109细胞/ L）。结论虽然常规的i-PRF方案可将血小板产量提高2至3倍，将白细胞产量提高50％，但我们有说服力地证明了通过收获0.3-0可将血小板和白细胞浓缩至10倍以上的能力。根据L-PRF规程，在血沉棕黄层中含有5 mL C-PRF。临床相关性先前的研究表明，i-PRF中血小板和白细胞的浓度仅略有增加。本研究描述了一种新颖的收获技术，其血小板和白细胞增加了10倍以上，可进一步用于组织再生。

"点击查看英文标题和摘要"

A novel method for harvesting concentrated platelet-rich fibrin (C-PRF) with a 10-fold increase in platelet and leukocyte yields.

BACKGROUND AND OBJECTIVES Liquid platelet rich fibrin (PRF; often referred to as injectable PRF) has been utilized as an injectable formulation of PRF that is capable of stimulating tissue regeneration. Our research group recently found that following standard L-PRF protocols (2700 RPM for 12 min), a massive increase in platelets and leukocytes was observed directly within the buffy-coat layer directly above the red blood cell layer. The purpose of this study was to develop a novel harvesting technique to isolate liquid PRF directly from this buffy coat layer and to compare this technique to standard i-PRF. MATERIALS AND METHODS Standard high g-force L-PRF and low g-force i-PRF protocols were utilized to separate blood layers. Above the red blood corpuscle layer, sequential 100-μL layers of plasma were harvested (12 layers total; i.e., 1.2 mL, which represents the total i-PRF volume), and 3 layers (3 × 100 μL) were harvested from the red blood cell layer to quantify blood cells. Each layer was then sent for complete blood count (CBC) analysis, and the cell numbers were quantified including red blood cells, leukocytes, neutrophils, lymphocytes, monocytes, and platelets. The liquid PRF that was directly collected from the buffy-coat layer following L-PRF protocols was referred to as concentrated PRF (C-PRF). RESULTS The i-PRF protocol typically yielded a 2- to 3-fold increase in platelets and a l.5-fold increase in leukocyte concentration from the 1- to 1.2-mL plasma layer compared to baseline concentrations in whole blood. While almost no cells were found in the first 4-mL layer of L-PRF, a massive accumulation of platelets and leukocytes was found directly within the buffy coat layer demonstrating extremely high concentrations of cells in this 0.3-0.5-mL layer (~ 20-fold increases). We therefore proposed harvesting this 0.3- to 0.5-mL layer directly above the red blood cell corpuscle layer as liquid C-PRF. In general, i-PRF was able to increase platelet numbers by ~ 250%, whereas a 1200-1700% increase in platelet numbers could easily be achieved by harvesting this 0.3-0.5 mL of C-PRF (total platelet concentrations of > 2000-3000 × 109 cells/L). CONCLUSION While conventional i-PRF protocols increase platelet yield by 2-3-fold and leukocyte yield by 50%, we convincingly demonstrated the ability to concentrate platelets and leukocytes over 10-fold by harvesting the 0.3-0.5 mL of C-PRF within the buffy coat following L-PRF protocols. CLINICAL RELEVANCE Previous studies have demonstrated only a slight increase in platelet and leukocyte concentrations in i-PRF. The present study described a novel harvesting technique with over a 10-fold increase in platelets and leukocytes that can be further utilized for tissue regeneration.

更新日期：2019-11-01

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