针灸减轻了啮齿动物酸和嘌呤引起的疼痛。,British Journal of Pharmacology

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针灸减轻了啮齿动物酸和嘌呤引起的疼痛。
British Journal of Pharmacology ( IF 6.8 ) Pub Date : 2019-11-21 , DOI: 10.1111/bph.14847
Ying Zhang ₁ , Lumei Huang ₁ , Sergey A Kozlov ₂ , Patrizia Rubini ₁ , Yong Tang ₁ , Peter Illes _{1,

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Affiliation

背景与目的我们着手研究在急性和炎症性啮齿动物疼痛模型中，电针和艾灸对一系列酸敏感和ATP敏感伤害感受器介导的疼痛状态的影响。实验方法我们将PBS（pH 6.0或4.0）或α，β-亚甲基ATP注射到大鼠或小鼠的爪中，引起热超敏反应，可通过爪退缩潜伏期（PWL）进行定量。通过将完全的弗氏佐剂（CFA）注射到大鼠的爪子中诱发炎症性疼痛。关键结果EAP和艾灸抵消了酸敏感离子通道3（ASIC3）和瞬时受体电位香草酸1（TRPV1）通道对pH 6.0 PBS的响应所引起的PWL的降低，但不能仅由TRPV1通道介导的PWL的降低注射pH 4.0 PBS。同样，EAP和艾灸预防了由嘌呤引起的疼痛，该疼痛是由P2X3（P2X2 / 3）和P2X7受体的刺激引起的。EAP和艾灸也减轻了CFA的作用。结论和意义在急性热痛和CFA引起的炎症性疼痛期间，ASIC3 / TRPV1通道和P2X3 / P2X7受体分别被质子和外源性α，β-亚甲基ATP或内源性ATP激活。EAP /艾灸可以逆转由ASIC3 / TRPV1通道激活介导的低阈值急性酸性疼痛，而不能由仅由TRPV1通道激活介导的高阈值酸性疼痛。ASIC3和P2X3受体似乎通过形成对EAP /艾灸敏感的ASIC3 / P2X3“同源受体”而对质子和ATP相互作用。

"点击查看英文标题和摘要"

Acupuncture alleviates acid- and purine-induced pain in rodents.

BACKGROUND AND PURPOSE We set out to investigate the effects of electroacupuncture (EAP) and moxibustion on pain states mediated by a range of acid- and ATP-sensitive nociceptors in acute and inflammatory rodent pain models. EXPERIMENTAL APPROACH We injected PBS (pH 6.0 or 4.0) or α,β-methylene ATP into the paw of rats or mice to cause thermal hypersensitivity, which was quantified by the paw withdrawal latency (PWL). Inflammatory pain was induced by the injection of complete Freund's adjuvant (CFA) into the rat paw. KEY RESULTS EAP and moxibustion counteracted the decrease of PWL mediated by acid-sensing ion channel 3 (ASIC3) and transient receptor potential vanilloid 1 (TRPV1) channels in response to pH 6.0 PBS, but not that mediated by TRPV1 channels only, initiated by the injection of pH 4.0 PBS. Similarly, EAP and moxibustion prevented the purinergically induced pain which was caused by stimulation of P2X3 (P2X2/3) and P2X7 receptors. The effect of CFA was also relieved by EAP and moxibustion. CONCLUSIONS AND IMPLICATIONS During acute thermal pain and CFA-induced inflammatory pain, ASIC3/TRPV1 channels and P2X3/P2X7 receptors are activated by protons and exogenous α,β-methylene ATP or endogenously released ATP respectively. Low-threshold acute acidic pain mediated by the activation of ASIC3/TRPV1 channels was reversed by EAP/moxibustion, while high-threshold acidic pain that is mediated exclusively by the activation of TRPV1 channels was not. ASIC3 and P2X3 receptors appear to interact with each other in responding to both protons and ATP, by forming an ASIC3/P2X3 “cognate receptor”, sensitive to EAP/moxibustion.

更新日期：2019-11-01

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