We assessed the effect of vitamin E and vitamin B (Vit E & Vit B) and their combination on sperm functional parameters in the rat varicocele model. Male rats (n = 120) were divided into control (n = 30), sham (n = 30) and varicocele induction (n = 60) groups. After 2 months, 10 rats from each group were sacrificed to verify varicocele model. This part of results showed that sperm parameters, DNA damage, lipid peroxidation and residual histone were adversely effected in the varicocele group. From the 50 remaining rats in varicocele group, 10 rats received Vit B complex (6, 9.6, 30.4, 9.6 and 0.006 mg/kg for B6, B2, B9, B1 and B12 respectively), 10 rats received Vit E (40 mg/kg), 10 rats received Vit B & E, 10 rats only received water and 10 rats were only received sesame oil as a solvent for Vit E, for 2 months. From 40 remaining rats in control and sham groups, 20 rats only received water and other 20 rats only received sesame oil for 2 months. Then, all the aforementioned parameters were assessed. These results showed that Vit B antioxidant was more efficient in improvement of sperm parameters, chromatin integrity and lipid peroxidation in varicocelized rats compared with Vit E.

中文翻译：

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维生素E和B对大鼠精索静脉曲张模型精子功能的影响。

我们在大鼠精索静脉曲张模型中评估了维生素E和维生素B（维生素E和维生素B）及其组合对精子功能参数的影响。将雄性大鼠（n ＝ 120）分为对照组（n ＝ 30），假（n ＝ 30）和精索静脉曲张诱导（n ＝ 60）组。2个月后，处死每组10只大鼠以验证精索静脉曲张模型。这部分结果表明精索静脉曲张组精子参数，DNA损伤，脂质过氧化和残留组蛋白均受到不利影响。在精索静脉曲张组的其余50只大鼠中，有10只大鼠接受了Vit B复合物（B6，B2，B9，B1和B12分别为6、9.6、30.4、9.6和0.006 mg / kg），有10只大鼠接受了Vit E（40 mg / kg kg），10只大鼠接受Vit B＆E，10只大鼠仅接受水，而10只大鼠仅接受芝麻油作为Vit E的溶剂，为期2个月。在对照组和假手术组的其余40只大鼠中，有20只只喝水，而另外20只只用麻油了2个月。然后，评估所有上述参数。这些结果表明，与Vit E相比，Vit B抗氧化剂在精索静脉曲张大鼠中改善精子参数，染色质完整性和脂质过氧化作用更有效。