垂体腺瘤研究的常用工具：细胞系和原代细胞。,Pituitary

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垂体腺瘤研究的常用工具：细胞系和原代细胞。
Pituitary ( IF 3.3 ) Pub Date : 2019-11-14 , DOI: 10.1007/s11102-019-01003-4
Ziyan Zhu ₁ , Weiwei Cui ₁ , Dimin Zhu ₂ , Nailin Gao ₂ , Yonghong Zhu ₁

Affiliation

目的

垂体瘤是人类常见的原发性脑瘤。为了进一步研究垂体腺瘤的发病机理和新的治疗靶标，细胞系和原代细胞是必要的工具。与具有短存活时间和激素分泌维持时间的原代细胞不同，在基因修饰的帮助下，细胞系将具有永生的特性。这篇综述旨在探讨这些细胞系在垂体腺瘤细胞中是否仍具有相似的病理生理变化，以及延长垂体腺瘤原代细胞寿命的方法。

结果

在本综述总结的细胞系中，HP75，PDFS，HPA和GX来源于人垂体腺瘤。发现2014年1月至2019年7月发表的文章中常用的细胞系是GH3，AtT20，MMQ，GH4C1，HP75和TtT / GF。此外，令人高兴的是，已经使用了许多方法来延长垂体腺瘤原代细胞的寿命并维持其特征。

结论

本文回顾了自1968年以来成功建立的大多数垂体腺瘤细胞系，以及垂体腺瘤细胞原代培养的相关情况。显然，这需要我们付出更多的努力来获得人垂体腺瘤细胞系，并在保持其形态和分泌激素能力的同时延长垂体腺瘤原代细胞的寿命。

"点击查看英文标题和摘要"

Common tools for pituitary adenomas research: cell lines and primary cells.

Purpose

Pituitary tumor is the common primary brain tumor in humans. For further studying the pathogenesis and new therapeutic targets of pituitary adenoma, cell lines and primary cells are necessary tools. Different from primary cells that have short survival time and hormone secretion maintenance time, cell lines would be endowed with immortal characteristics under the help of gene modification. This review is to explore whether these cell lines still have similar pathophysiological changes in pituitary adenoma cells and methods to prolong the lifespan of pituitary adenoma primary cells.

Results

In the cell lines summarized in the review, HP75, PDFS, HPA and GX were derived from human pituitary adenomas. It was found that the cell lines commonly used in articles published between January 2014 and July 2019 were GH3, AtT20, MMQ, GH4C1, HP75 and TtT/GF. Besides, it was glad that many methods had been used to prolong the lifespan and maintain characteristics of pituitary adenoma primary cells.

Conclusion

The paper reviews most of pituitary adenoma cell lines that have been successfully established since 1968 and the relevant situation of primary culture of pituitary adenoma cells. Obviously, it requires us to make more efforts to obtain human pituitary adenoma cell lines and prolong the lifespan of pituitary adenoma primary cells with maintaining their morphology and ability to secret hormones.

更新日期：2019-11-14

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