Protein & Peptide Letters ( IF 1.0 ) Pub Date : 2020-05-31 , DOI: 10.2174/0929866526666191112152646 Vibhor Mishra 1
The Baculovirus Expression Vector System (BEVS) is a workhorse for recombinant protein expression for over thirty-five years. Ever since it was first used to overexpress the human IFN-β protein, the system has been engineered and modified several times for quick and easy expression and scale-up of the recombinant proteins. Multiple gene assemblies performed on the baculovirus genome using synthetic biology methods lead to optimized overexpression of the multiprotein complexes. Nowadays, several commercially available BEVS platforms offer a variety of customizable features, and often it is confusing which one to choose for a novice user. This short review is intended to be a one-stop guide to the commercially available baculovirus technology for heterologous protein expression in the insect cells, which users can refer to choose from popular and desirable BEVS products or services.
中文翻译:
用于重组蛋白生产的商业杆状病毒表达载体系统的综合指南。
杆状病毒表达载体系统(BEVS)是用于重组蛋白表达的重要工具,已有超过35年的历史。自从首次将它用于过表达人IFN-β蛋白以来,就对该系统进行了多次工程设计和修改,以快速,轻松地表达和扩大重组蛋白的规模。使用合成生物学方法对杆状病毒基因组进行的多个基因组装可导致多蛋白复合物的优化过表达。如今,一些商业上可买到的BEVS平台提供了各种可自定义的功能,对于新手用户来说,选择哪个功能常常令人困惑。这篇简短的评论旨在为昆虫病毒中异源蛋白表达的杆状病毒技术提供一站式指导,