拟南芥转录因子RAV1和RAV2由与触摸相关的刺激以剂量依赖性和双相方式调节。,Genes & Genetic Systems

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拟南芥转录因子RAV1和RAV2由与触摸相关的刺激以剂量依赖性和双相方式调节。
Genes & Genetic Systems Pub Date : 2009-05-08 , DOI: 10.1266/ggs.84.95
Yasuaki Kagaya ₁ , Tsukaho Hattori

Affiliation

来自拟南芥的转录因子RAV1和RAV2包含两个不同的DNA结合结构域AP2 / EREBP和B3，两者在植物中都是独特的。我们发现，RAV1和RAV2的转录本被触摸相关的机械刺激瞬时上调。但是，RAV1和RAV2的时间表达模式与已知的触摸诱导基因不同。机械刺激诱导的RAV1和RAV2表达的显着特征是它是双相的。快速和短暂的诱导后，RAV1和RAV2的表达被还原并维持。触摸刺激的瞬时和随后上调的程度取决于初始刺激的剂量。转基因A的分析携带RAV2启动子-GUS融合基因的拟南芥植物表明，短暂的机械刺激诱导的RAV2表达主要受其启动子控制。对转基因植物的组织化学分析表明，在真叶和茎尖分生组织的叶柄和原基中强烈诱导了GUS表达，这可能与触摸刺激引起的植物生长方式改变有关。由于RAV1被认为是生长和发育的负调节剂，RAV1和RAV2的剂量依赖性双相上调不仅可用于即时的生理反应，而且还可用于响应环境刺激而适应发育。对转基因植物的组织化学分析表明，在真叶和茎尖分生组织的叶柄和原基中强烈诱导了GUS表达，这可能与触摸刺激引起的植物生长方式改变有关。由于RAV1被认为是生长和发育的负调节剂，RAV1和RAV2的剂量依赖性双相上调不仅可用于即时的生理反应，而且还可用于响应环境刺激而适应发育。对转基因植物的组织化学分析表明，在真叶和茎尖分生组织的叶柄和原基中强烈诱导了GUS表达，这可能与触摸刺激引起的植物生长方式改变有关。由于RAV1被认为是生长和发育的负调节剂，RAV1和RAV2的剂量依赖性双相上调不仅可用于即时的生理反应，而且还可用于响应环境刺激而适应发育。

"点击查看英文标题和摘要"

Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner.

Transcription factors, RAV1 and RAV2 from Arabidopsis thaliana, contain two distinct DNA-binding domains, AP2/EREBP and B3, both of which are uniquely found in plants. We found that transcripts of RAV1 and RAV2 were upregulated transiently by touch-related mechanical stimuli. However, the temporal expression patterns of RAV1 and RAV2 differed from those of known touch-induced genes. A striking feature of mechanical stimulus-induced expression of RAV1 and RAV2 was that it was biphasic; the RAV1 and RAV2 expression was reinduced and sustained after a rapid and transient induction. The extent of both transient and subsequent upregulation by touch-stimuli depended on the dose of the initial stimulus. Analysis of transgenic A. thaliana plants carrying a RAV2 promoter-GUS fusion gene indicated that the transient mechanical stimulus-induced RAV2 expression was primarily controlled by its promoter. Histochemical analysis of the transgenic plants revealed that GUS expression was strongly induced in the petioles and primordia of true leaves and shoot apical meristems, which may be related to the alteration in plant growth pattern caused by touch-stimuli. Because RAV1 has been suggested to be a negative regulator of growth and development, the dose-dependent biphasic upregulation of RAV1 and RAV2 may serve not only for immediate physiological responses and but also for developmental adaptation in response to the environmental stimuli.

更新日期：2019-11-01

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