Gomisin M2 isolated from Schisandra viridis A. C. Smith has potential anti-tumor effects on certain cancers, including breast cancer. However, only a few investigations have been conducted on the effects of Gomisin M2 on breast cancer stem cells (CSCs), which have the ability to self-renew and differentiate, as a possible strategy to resolve cancer cell resistance to apoptosis and to improve treatments. It is essential to investigate the effects of Gomisin M2 on breast cancer stem cells (BCSCs). In this study, we enriched breast cancer stem cells with CD44+/CD24- from MDA-MB-231 and HCC1806 cells through magnetic-activated cell sorting and cultured these in serum-free medium. The ability of Gomisin M2 to kill breast cancer stem cells was evaluated in vitro and in vivo. Gomisin M2 significantly inhibited the proliferation of the triple-negative breast cancer cell lines and mammosphere formation in breast CSCs and downregulated the Wnt/β-catenin self-renewal pathway. Moreover, Gomisin M2 induced apoptosis and blocked the mitochondrial membrane potential of BCSCs. Gomisin M2 suppressed the proliferation of MDA-MB-231 and HCC1806 xenografts in zebrafish. Together, these findings suggest that the anti-BCSC activity of Gomisin M2 could become a promising starting point for the discovery of novel BCSC-targeting drugs.

中文翻译：

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来自Baizuan的Gomisin M2在斑马鱼异种移植模型中抑制乳腺癌干细胞增殖。

从五味子五味子AC Smith分离出的Gomisin M2对某些癌症（包括乳腺癌）具有潜在的抗肿瘤作用。然而，关于Gomisin M2对乳腺癌干细胞（CSCs）的作用仅进行了很少的研究，这些干细胞具有自我更新和分化的能力，可以作为解决癌细胞对凋亡的抗性并改善治疗方法的策略。研究Gomisin M2对乳腺癌干细胞（BCSCs）的影响至关重要。在这项研究中，我们通过磁激活细胞分选法从MDA-MB-231和HCC1806细胞中富集了CD44 + / CD24-乳腺癌干细胞，并在无血清培养基中进行了培养。在体外和体内评估了Gomisin M2杀死乳腺癌干细胞的能力。Gomisin M2显着抑制乳腺癌CSC中三阴性乳腺癌细胞系的增殖和乳球形成，并下调Wnt /β-catenin的自我更新途径。此外，Gomisin M2诱导细胞凋亡并阻断BCSC的线粒体膜电位。Gomisin M2抑制了斑马鱼中MDA-MB-231和HCC1806异种移植物的增殖。总之，这些发现表明，Gomisin M2的抗BCSC活性可能成为发现新型靶向BCSC的药物的有希望的起点。Gomisin M2抑制了斑马鱼中MDA-MB-231和HCC1806异种移植物的增殖。总之，这些发现表明，Gomisin M2的抗BCSC活性可能成为发现新型靶向BCSC的药物的有希望的起点。Gomisin M2抑制了斑马鱼中MDA-MB-231和HCC1806异种移植物的增殖。总之，这些发现表明，Gomisin M2的抗BCSC活性可能成为发现新型靶向BCSC的药物的有希望的起点。