The rare secondary flavonol glycoside Sagittatoside A has much better in vivo bioactivities than epimedin A in Epimedii Folium. However, its current preparation methods are of low efficiency, with byproducts generated. The aim of this study was to establish a novel catalysis system for effective and convenient preparation of Sagittatoside A from epimedin A based on recyclable and integrated aqueous organic two-phase enzymatic hydrolysis. The system was consisted of propyl acetate and HAc-NaAc buffer (pH4.5) containing β-dextranase/epimedin A, and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin A was completely hydrolyzed to sagittatoside A, and 95.02% of the product was transferred into the organic phase. Moreover, 90% of its initial activity was retained after seven cycles of hydrolysis. Additionally, the procedure was simpler than conventional enzymatic hydrolysis. Collectively, the newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside A in industrial application.

稀有的次黄酮总糖苷人参皂甙A具有比Epimedii Folium中的Epimedin A更好的体内生物活性。然而，其目前的制备方法效率低下，并产生副产物。这项研究的目的是建立一种新型的催化体系，该体系基于可回收和整合的有机有机水两相酶水解作用，从Epimedin A有效而方便地制备SagittatosideA。该系统由乙酸丙酯和含β的HAc-NaAc缓冲液（pH4.5）组成-葡聚糖酶/表环丁素A，并且在60℃下进行水解1小时。结果，表柔比丁A被完全水解成箭叶苷A，并且95.02％的产物被转移到有机相中。此外，在七个水解周期后保留了其90％的初始活性。另外，该过程比常规酶水解更简单。总的来说，新提出的策略是一种在工业应用中制备人参皂苷A的有效方法。