通过低速概念优化富含血小板的纤维蛋白：生长因子释放，生物相容性和细胞反应。,Journal of Periodontology

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通过低速概念优化富含血小板的纤维蛋白：生长因子释放，生物相容性和细胞反应。
Journal of Periodontology ( IF 4.2 ) Pub Date : 2016-09-03 , DOI: 10.1902/jop.2016.160443
Masako Fujioka-Kobayashi _{1,

2,

3} , Richard J Miron ₁ , Maria Hernandez ₁ , Umadevi Kandalam ₄ , Yufeng Zhang ₅ , Joseph Choukroun ₆

Affiliation

背景技术在过去的十年中，作为一种用于组织再生的低成本纤维蛋白基质，使用白细胞富含血小板的纤维蛋白（L-PRF）在再生牙科领域获得了巨大的发展势头。这项研究表征了离心速度（G力）以及离心时间如何影响从纤维蛋白凝块中释放生长因子，以及暴露于每种PRF基质的牙龈成纤维细胞的细胞活性。方法以标准L-PRF作为对照（每分钟[2,700转[rpm] -12分钟]）。研究了两个测试组，分别使用低速（1300 rpm-14分钟，称为高级PRF [A-PRF]）和低速+时间（1300 rpm-8分钟； A-PRF +）。测试每种PRF基质长达10天的生长因子释放（八个供体样品）以及生物相容性和细胞活性。结果低速概念（A-PRF，A-PRF +）证明血小板衍生生长因子（PDGF），转化生长因子（TGF）-β1，表皮生长因子和胰岛素样生长因子的生长因子释放显着增加，其中A-PRF +最高组。尽管所有PRF制剂由于其自身来源而具有极高的生物相容性，但与L-PRF相比，A-PRF和A-PRF +均显示出明显更高的人类成纤维细胞迁移和增殖水平。此外，用A-PRF +培养的牙龈成纤维细胞在3天或7天时，PDGF，TGF-β和胶原蛋白1的信使RNA（mRNA）水平显着提高。结论本研究的结果表明，低速概念对离心速度和时间的改进有利于增加PRF血块中生长因子的释放。反过来，可能通过增加成纤维细胞迁移，增殖和胶原mRNA水平直接影响组织再生。现在有必要进行进一步的动物和临床研究。

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Optimized Platelet-Rich Fibrin With the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response.

BACKGROUND Over the past decade, use of leukocyte platelet-rich fibrin (L-PRF) has gained tremendous momentum in regenerative dentistry as a low-cost fibrin matrix used for tissue regeneration. This study characterizes how centrifugation speed (G-force) along with centrifugation time influence growth factor release from fibrin clots, as well as the cellular activity of gingival fibroblasts exposed to each PRF matrix. METHODS Standard L-PRF served as a control (2,700 revolutions per minute [rpm]-12 minutes). Two test groups using low-speed (1,300 rpm-14 minutes, termed advanced PRF [A-PRF]) and low-speed + time (1,300 rpm-8 minutes; A-PRF+) were investigated. Each PRF matrix was tested for growth factor release up to 10 days (eight donor samples) as well as biocompatibility and cellular activity. RESULTS The low-speed concept (A-PRF, A-PRF+) demonstrated a significant increase in growth factor release of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-β1, epidermal growth factor, and insulin-like growth factor, with A-PRF+ being highest of all groups. Although all PRF formulations were extremely biocompatible due to their autogenous sources, both A-PRF and A-PRF+ demonstrated significantly higher levels of human fibroblast migration and proliferation compared with L-PRF. Furthermore, gingival fibroblasts cultured with A-PRF+ demonstrated significantly higher messenger RNA (mRNA) levels of PDGF, TGF-β, and collagen1 at either 3 or 7 days. CONCLUSIONS The findings from the present study demonstrate modifications to centrifugation speed and time with the low-speed concept favor an increase in growth factor release from PRF clots. This, in turn, may directly influence tissue regeneration by increasing fibroblast migration, proliferation, and collagen mRNA levels. Future animal and clinical studies are now necessary.

更新日期：2019-11-01

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