N,N-Dimethylaniline (DMA, CAS No. 121-69-7) and N,N-dimethyl-p-toluidine (DMPT, CAS No. 99-97-8) belong to the N-dialkylaminoaromatics, a chemical class structurally alerting to DNA reactivity. Their applications may be industrial (dye and pesticide intermediates, polymerizing agents) and surgical (polymerization accelerators for the manufacture of bone cements and prosthetic devices), thus implying heterogeneous types of human exposure. Findings of carcinogenicity in rodents and some nonexhaustive genotoxicity data are available for DMA, but to our knowledge no information is available on DMPT concerning either carcinogenicity or any kind of genetic toxicity. To investigate their mechanism of action and mutagenic/carcinogenic potential, DMA and DMPT were analyzed for complementary genotoxicity endpoints, namely, gene mutation in Salmonella (Ames test), structural and numerical chromosome aberrations in hamster V79 cells (micronucleus test, matched with an immunofluorescent staining for kinetochore proteins), and in vivo DNA damage in mouse and rat liver (alkaline DNA elution test). The results essentially indicate that both chemicals are chromosome damaging agents. Indeed, at the maximum nontoxic doses, they proved nonmutagenic in Salmonella (although their toxicity did not allow concentrations > 70 micrograms/plate to be tested) and weakly positive in inducing DNA damage (increases in DNA elution rates at most approximately 2.4 times control value). Conversely, they proved clearly positive in inducing numerical chromosome alterations, with dose-dependent increases up to more than five times the control value for DMPT. At the highest dose tested, both chemicals also showed a significant clastogenic effect.

中文翻译：

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N，N-二甲基苯胺和N，N-二甲基-对甲苯胺的遗传毒性分析。

N，N-二甲基苯胺（DMA，CAS No. 121-69-7）和N，N-二甲基对甲苯胺（DMPT，CAS No. 99-97-8）属于N-二烷基氨基芳族化合物，在结构上属于化学类别提醒DNA反应性。它们的应用可能是工业的（染料和农药中间体，聚合剂）和外科的（用于制造骨水泥和修复设备的聚合促进剂），因此暗示了人类接触的不同类型。关于DMA的啮齿动物有致癌性的发现和一些非详尽的遗传毒性数据，但据我们所知，关于致癌性或任何种类的遗传毒性，没有关于DMPT的信息。为了研究其作用机理和诱变/致癌潜力，分析了DMA和DMPT的互补遗传毒性终点，即 沙门氏菌的基因突变（Ames试验），仓鼠V79细胞的结构和数值染色体畸变（微核试验，以及针对线粒体蛋白的免疫荧光染色）以及小鼠和大鼠肝脏的体内DNA损伤（碱性DNA洗脱试验）。结果实质上表明这两种化学物质都是染色体破坏剂。的确，在最大无毒剂量下，它们被证明在沙门氏菌中没有致突变性（尽管其毒性不允许浓度> 70微克/板进行测试），并且在诱导DNA损伤方面呈弱阳性（DNA洗脱速率最多增加至对照值的2.4倍）。 ）。相反，它们被证明在诱导染色体数字改变方面明显是阳性的，剂量依赖性增加高达DMPT对照值的五倍以上。在测试的最高剂量下，