An enzymatic method for synthesis of labelled thyroxine glucuronide (T4G) and triiodothyronine glucuronide (T3G) from labelled thyroxine (T4) and triiodothyronine (T3) is presented. The synthetic glucuronides are completely digested by beta-glucuronidase, with recovery of the parent T4 or T3. They have distinctive elution patterns on HPLC and on Sephadex G25 chromatography, and can be clearly separated from T4 and T3 as well as from synthetic T4 sulfate (T4S) and T3 sulfate (T3S). On LH 20 chromatography, elution of T4G and T3G is intermediate between that of T4 and T3 and that of T4S and T3S. T3G can be well separated from other thyronines by HPLC alone, but T4G coelutes with rT3 on HPLC; these are then separated by adding a Sephadex G25 chromatography step. Biosynthetic 131I-T3G and 125I-T4G from the bile of a cat given 131I-T3 and 125I-T4 had similar HPLC chromatographic patterns to those of synthetic T3G and T4G. That the identified peaks from analysis of the bile were indeed T3G and T4G was confirmed by recovery of the parent T3 and T4 after beta-glucuronidase digestion.

中文翻译：

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甲状腺素和三碘甲状腺素的葡糖醛酸苷和硫酸盐结合物的制备和分离。

提出了一种从标记的甲状腺素（T4）和三碘甲状腺素（T3）合成标记的甲状腺素葡萄糖醛酸（T4G）和三碘甲状腺素（T3G）的酶促方法。合成的葡糖醛酸苷被β-葡糖醛酸苷酶完全消化，同时回收了母体T4或T3。它们在HPLC和Sephadex G25色谱上具有独特的洗脱模式，并且可以与T4和T3以及合成的T4硫酸盐（T4S）和T3硫酸盐（T3S）清楚地分离。在LH 20色谱上，T4G和T3G的洗脱介于T4和T3的洗脱与T4S和T3S的洗脱之间。仅通过HPLC即可将T3G与其他甲状腺素很好地分离，但在HPLC上T4G与rT3共洗脱。然后通过添加Sephadex G25色谱分离步骤将其分离。给予131I-T3和125I-T4的猫胆汁的生物合成131I-T3G和125I-T4G具有与合成T3G和T4G相似的HPLC色谱图。通过β-葡萄糖醛酸苷酶消化后母体T3和T4的回收，确认了通过胆汁分析鉴定的峰确实是T3G和T4G。