4-Fluorobenzenesulfonyl chloride (fosyl chloride), due to the strong electron-withdrawing property of its fluoride atom, is found to be an excellent activating agent for the covalent attachment of biologicals to a variety of solid supports (e.g. functionalized polystyrene microspheres, Sepharose beads, or cellulose rods and hollow fibers). This reagent reacts rapidly with primary or secondary hydroxyl groups, at ambient temperature and pressure, to form 4-fluorobenzenesulfonate leaving groups. The activated solid support can be used immediately or preserved for several months without loss of activity by freeze-drying or by storage at 4 degrees C in aqueous solution at pH 5. Enzymes, antibodies, avidin, and other biologicals can be covalently attached to the activated solid phase with excellent retention of biological function. Potential therapeutic applications of the fosyl chloride chemistry for bioselective separation of human lymphocyte subsets from whole blood and tumor cells from bone marrow are presented.

中文翻译：

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使用4-氟苯磺酰氯活化聚合物载体的羟基。

由于4-氟苯磺酰氯（fosyl chloride）的氟原子具有很强的吸电子特性，因此被发现是将生物物质共价附着于各种固体支持物（例如功能化的聚苯乙烯微球，琼脂糖凝胶珠子）的出色活化剂。 ，或纤维素棒和中空纤维）。该试剂在环境温度和压力下与伯或仲羟基快速反应，形成4-氟苯磺酸盐离去基团。活化的固体支持物可立即使用或保存几个月，而不会因冷冻干燥或在4℃的pH 5水溶液中保存而失去活性。酶，抗体，抗生物素蛋白和其他生物可共价结合于其上。活化的固相，具有出色的生物学功能。