Rhodojaponin III is a bioactive diterpenoid isolated from the medicinal plant Rhododendron molle G. Don. Quantitative analysis of rhodojaponin III was challenging and the pharmacokinetics of oral rhodojaponin III remained to be investigated. Here, a rapid and sensitive liquid chromatography tandem mass spectrometric (LC-MS/MS) method was developed and validated. The calibration curve was linear over the concentration range of 1-200 ng/mL (r = 0.992). The method was further validated following internationally approved guidelines and all the issues including intra- and inter-day precision, accuracy, carryover, extraction recovery, matrix effects and stability met the recommended limits. The method was then applied to study the pharmacokinetics of rhodojaponin III in mice after intravenous (0.06 mg/kg) or oral (0.24 mg/kg) administration. The results showed that rhodojaponin III had fast oral absorption (time to peak concentration, 0.08 h) and good oral bioavailability (73.6%). In addition, rhodojaponin III was quickly eliminated after it was intravenously or orally administered, with half-life values of 0.19 and 0.76 h, respectively. After oral administration, it was widely distributed in tissues including kidney, lung, heart, spleen and thymus, but had extremely low concentrations in liver and brain. The data presented in this study is beneficial for the further study of rhodojaponin III.

中文翻译：

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使用QTRAP LC-MS / MS在大鼠体内对杜鹃皂苷III进行药代动力学，生物利用度和组织分布研究。

Rhodojaponin III是从药用植物Rhododendron molle G. Don分离的生物活性二萜。杜鹃花皂苷III的定量分析具有挑战性，口服杜鹃花皂苷III的药代动力学仍有待研究。在此，开发并验证了快速灵敏的液相色谱串联质谱（LC-MS / MS）方法。校准曲线在1-200 ng / mL的浓度范围内呈线性（r = 0.992）。该方法已按照国际认可的准则进行了进一步验证，并且日间和日间精度，准确度，残留，提取回收率，基质效应和稳定性等所有问题均达到建议的极限。然后将该方法用于研究静脉内（0.06 mg / kg）或口服（0.24 mg / kg）施用的杜鹃皂甙III在小鼠中的药代动力学。结果表明，杜鹃皂苷III具有快速的口服吸收（峰值浓度时间为0.08 h）和良好的口服生物利用度（73.6％）。此外，静脉注射或口服后，杜鹃皂甙III迅速消失，半衰期分别为0.19和0.76 h。口服后，它广泛分布在包括肾脏，肺，心脏，脾脏和胸腺的组织中，但在肝脏和大脑中的含量极低。本研究中提供的数据有助于进一步研究杜鹃皂苷III。口服后，它广泛分布在包括肾脏，肺，心脏，脾脏和胸腺的组织中，但在肝脏和大脑中的含量极低。本研究中提供的数据有助于进一步研究杜鹃皂苷III。口服后，它广泛分布在包括肾脏，肺，心脏，脾脏和胸腺的组织中，但在肝脏和大脑中的含量极低。本研究中提供的数据有助于进一步研究杜鹃皂苷III。