卵巢癌患者的生存与腹水中是否存在 DC 和 T 细胞亚群无关。,Frontiers in Immunology

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卵巢癌患者的生存与腹水中是否存在 DC 和 T 细胞亚群无关。
Frontiers in Immunology ( IF 5.7 ) Pub Date : 2019-01-11 , DOI: 10.3389/fimmu.2018.03156
Christina Wefers _{1,

2} , Tjitske Duiveman-de Boer ₁ , Refika Yigit _{1,

2} , Petra L M Zusterzeel ₂ , Anne M van Altena ₂ , Leon F A G Massuger ₂ , I Jolanda M De Vries ₁

Affiliation

腹水是卵巢癌的一个显着特征，可以作为液体活检来评估患者的免疫状态。肿瘤浸润 T 淋巴细胞与卵巢癌生存率的提高相关。为了研究腹水中的免疫细胞是否与患者的预后相关，我们分析了诊断为高级别浆液性癌 (HGSC) 的卵巢癌患者腹水中树突状细胞 (DC) 和 T 细胞亚群的数量。化疗前从 62 名 HGSC 患者身上收集腹水。收集患者的临床病理学、组织学和随访数据。使用密度梯度离心分离腹水来源的免疫细胞。使用流式细胞术分析骨髓 DC（BDCA-1+、BDCA-3+、CD16+）、pDC（CD123+BDCA-2+）和 T 细胞（CD4+、CD8+）的存在。完全细胞减灭术、对主要治疗的反应和化疗敏感性与患者预后的改善相关。相比之下，腹水中的免疫细胞与患者的生存率没有显着相关性。然而，我们观察到 CD4+ T 细胞百分比较低的患者的预后有改善的趋势。此外，我们评估了 10 个腹水样本中 T 细胞和非免疫细胞上共刺激和共抑制分子的表达。30% 的腹水来源 T 细胞表达 PD-1，50% 的非免疫细胞表达 PD-L1。然而，腹水中DC和T细胞亚群的百分比与HGSC患者的存活率并不直接相关。

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Survival of Ovarian Cancer Patients Is Independent of the Presence of DC and T Cell Subsets in Ascites.

Ascites is a prominent feature of ovarian cancer and could serve as liquid biopsy to assess the immune status of patients. Tumor-infiltrating T lymphocytes are correlated with improved survival in ovarian cancer. To investigate whether immune cells in ascites are associated with patient outcome, we analyzed the amount of dendritic cell (DC) and T cell subsets in ascites from ovarian cancer patients diagnosed with high-grade serous cancer (HGSC). Ascites was collected from 62 HGSC patients prior to chemotherapy. Clinicopathological, histological and follow-up data from patients were collected. Ascites-derived immune cells were isolated using density-gradient centrifugation. The presence of myeloid DCs (BDCA-1+, BDCA-3+, CD16+), pDCs (CD123+BDCA-2+), and T cells (CD4+, CD8+) was analyzed using flow cytometry. Complete cytoreduction, response to primary treatment and chemosensitivity were associated with improved patient outcome. In contrast, immune cells in ascites did not significantly correlate with patient survival. However, we observed a trend toward improved outcome for patients having low percentages of CD4+ T cells. Furthermore, we assessed the expression of co-stimulatory and co-inhibitory molecules on T cells and non-immune cells in 10 ascites samples. PD-1 was expressed by 30% of ascites-derived T cells and PD-L1 by 50% of non-immune cells. However, the percentage of DC and T cell subsets in ascites was not directly correlated to the survival of HGSC patients.

更新日期：2019-11-01

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