Oosporein 是白僵菌中丰富的代谢物，具有反馈诱导机制和昆虫毒力作用,Fungal Biology

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Oosporein 是白僵菌中丰富的代谢物，具有反馈诱导机制和昆虫毒力作用
Fungal Biology ( IF 2.9 ) Pub Date : 2019-08-01 , DOI: 10.1016/j.funbio.2019.01.004
Louise Mc Namara ₁ , Stephen K Dolan ₂ , John M D Walsh ₃ , John C Stephens ₃ , Travis R Glare ₄ , Kevin Kavanagh ₅ , Christine T Griffin ₅

Affiliation

Oosporein 最早是在 50 年前从昆虫病原体 Beauveria bassiana 中发现的。在这里，我们研究了白僵菌产生的卵孢子素对林业害虫 Hylobius abietis 和模式昆虫 Galleria mellonella 的杀虫、拒食和免疫调节作用。我们报告了一种调节 B. caledonica 卵孢子素产生的新型反馈诱导机制；外源性卵孢子素诱导卵孢子素簇的表达，导致卵孢子素生物合成酶的丰度增加，如无标记定量蛋白质组学所示。Oosporein 对 H. abietis 成虫没有拒食作用——相反，接触 oosporein 处理过的食物的昆虫比只接触未经处理的食物的昆虫吃得更多。注射卵孢子素不会杀死昆虫幼虫，但会增加对幽门螺杆菌的易感性。abietis 到随后的感染。在测试的浓度下，卵孢子素对 H. abietis 成虫和 G. mellonella 幼虫没有起到接触毒素的作用。因此，似乎卵孢子素促进感染而不是直接杀死昆虫；这可以通过血细胞数量的减少和体液免疫系统的改变来介导。这项工作为未来研究 B. caledonica 作为生物防治剂的潜在用途提供了案例，通过与卵孢子素组合或提高卵孢子素的产量。这可以通过血细胞数量的减少和体液免疫系统的改变来介导。这项工作为未来研究 B. caledonica 作为生物防治剂的潜在用途提供了案例，通过与卵孢子素组合或提高卵孢子素的产量。这可以通过血细胞数量的减少和体液免疫系统的改变来介导。这项工作为未来研究 B. caledonica 作为生物防治剂的潜在用途提供了案例，通过与卵孢子素组合或提高卵孢子素的产量。

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Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence

Oosporein was first identified from the insect pathogen Beauveria bassiana >50 y ago. Here, we investigate the insecticidal, anti-feedant and immunomodulation effects of oosporein produced by Beauveria caledonica on the forestry pest Hylobius abietis and model insect Galleria mellonella. We report a novel feedback induction mechanism regulating oosporein production in B. caledonica; exogenous oosporein induces the expression of the oosporein cluster, leading to increased abundance of oosporein biosynthetic enzymes, as shown by label-free quantitative proteomics. Oosporein did not have an anti-feedant effect on H. abietis adults - on the contrary, insects exposed to oosporein-treated food fed more than those exposed to untreated food only. Injected oosporein did not kill insect larvae but increased susceptibility of H. abietis to a subsequent infection. Oosporein did not act as a contact toxin on H. abietis adults and G. mellonella larvae at the concentrations tested. Therefore, it appears that oosporein promotes infection rather than directly killing insects; this could be mediated both by a reduction in haemocyte numbers and by alterations to the humoral immune system. This work makes a case for future research into the potential use of B. caledonica as a biocontrol agent through combinations with oosporein or with enhanced production of oosporein.

更新日期：2019-08-01

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