In January 2018, the Drug Enforcement Agency temporarily designated cyclopropylfentanyl as a Schedule I drug. Over the course of 5 months (December 2017-May 2018), the Nassau County Medical Examiner Toxicology Laboratory qualitatively identified and confirmed cyclopropylfentanyl in specimens obtained from five postmortem cases. We describe the five cases and include pertinent autopsy findings and decedent histories, along with results for cyclopropylfentanyl determined in postmortem cardiac blood. Samples were prepared by an alkaline liquid-liquid extraction, with sample pH adjusted to >9 and utilizing an extraction solvent consisting of 90:10 hexane:ethyl acetate. Instrumental analysis was achieved via liquid chromatography tandem mass spectrometry with a dual jetstream electrospray source operating in positive ion mode. Two ion transitions were monitored for each analyte of interest and the internal standard. The estimated concentration range of cyclopropylfentanyl in the reported cases was 5.6 to 82 ng/mL for five postmortem cardiac blood specimens. All five cases included cyclopropylfentanyl in the established cause of death.

中文翻译：

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通过LC-MS-MS定性分析环丙基芬太尼的5个事后案例报告。

2018年1月，禁毒署将环丙基芬太尼临时指定为附表I药物。在5个月（2017年12月至2018年5月）的过程中，拿骚县医学检验员毒理学实验室定性鉴定并确认了从5个死后病例中获得的标本中的环丙基芬太尼。我们描述了这五种情况，包括相关的尸检结果和后遗的病史，以及死后心脏血液中确定的环丙基芬太尼结果。通过碱液-液萃取制备样品，其中样品pH调节至> 9，并使用由90:10己烷：乙酸乙酯组成的萃取溶剂。通过液相色谱串联质谱法以正离子模式运行的双喷射流电喷雾源实现仪器分析。监测每种目标分析物和内标物的两个离子跃迁。在五个死后的心脏血液样本中，报告的病例中环丙基芬太尼的估计浓度范围为5.6至82 ng / mL。所有五例病例均在确定的死亡原因中包括环丙基芬太尼。