两种具有克隆间表型异质性的急性单核细胞白血病（AML-M5a）细胞系ins（11; 9）（q23; p22p23），表现出由隐性染色体插入导致的MLL-AF9融合。,Leukemia

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两种具有克隆间表型异质性的急性单核细胞白血病（AML-M5a）细胞系ins（11; 9）（q23; p22p23），表现出由隐性染色体插入导致的MLL-AF9融合。
Leukemia ( IF 12.8 ) Pub Date : 1997-09-26 , DOI: 10.1038/sj.leu.2400768
Y Matsuo ₁ , R A MacLeod , C C Uphoff , H G Drexler , C Nishizaki , Y Katayama , G Kimura , N Fujii , E Omoto , M Harada , K Orita

Affiliation

我们描述了两种新的人类白血病细胞系MOLM-13和MOLM-14，它们是从急性单核细胞白血病FAB M5a复发时从患者外周血建立的，FAB M5a已从骨髓增生异常综合症（MDS）演变而来。两种细胞系均表达单核细胞特异性酯酶（MSE）和MLL-AF9融合mRNA。基因融合与微小的染色体插入ins（11; 9）（q23; p22p23）有关。MOLM-13和MOLM-14是通过染色体插入引起的MLL基因重排的第一个细胞系，代表了第三个报道的病例。两种细胞系都携带在MDS阶段也存在的三体性8，以及与t（9; 11）相关的最常见的三体性，即+ 6，+ 13，+ 19不同地存在于不同的亚克隆中。尽管具有这些共同点，但在两种细胞系之间仍注意到抗原表达的差异：MOLM-13的为CD34 +，CD13-，CD14-，CD15 +，CD33 +；而MOLM-14是CD4 +，CD13 +，CD14 +，CD15 +，CD33 +。单独用INF-γ刺激或与TNF-α联合刺激后，两种细胞系均可诱导巨噬细胞样形态分化，这种处理也诱导或上调了某些与骨髓单核细胞相关抗原的表达，包括CD13，CD14，CD15 ，CD64，CD65和CD87。在一起，这些数据证实这两种细胞系可能是研究单核细胞分化和白血病发生的体外模型。该治疗还诱导或上调了某些与骨髓单核细胞相关的抗原的表达，包括CD13，CD14，CD15，CD64，CD65和CD87。在一起，这些数据证实这两种细胞系可能是研究单核细胞分化和白血病发生的体外模型。该治疗还诱导或上调了某些与骨髓单核细胞相关的抗原的表达，包括CD13，CD14，CD15，CD64，CD65和CD87。在一起，这些数据证实这两种细胞系可能是研究单核细胞分化和白血病发生的体外模型。

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Two acute monocytic leukemia (AML-M5a) cell lines (MOLM-13 and MOLM-14) with interclonal phenotypic heterogeneity showing MLL-AF9 fusion resulting from an occult chromosome insertion, ins(11;9)(q23;p22p23).

We describe two new human leukemia cell lines, MOLM-13 and MOLM-14, established from the peripheral blood of a patient at relapse of acute monocytic leukemia, FAB M5a, which had evolved from myelodysplastic syndrome (MDS). Both cell lines express monocyte-specific esterase (MSE) and MLL-AF9 fusion mRNA. Gene fusion is associated with a minute chromosomal insertion, ins(11;9)(q23;p22p23). MOLM-13 and MOLM-14 are the first cell lines with, and represent the third reported case of, MLL gene rearrangement arising via chromosomal insertion. Both cell lines carry trisomy 8 which was also present during the MDS phase, as well as the most frequent trisomies associated with t(9;11), ie, +6, +13, +19 variously present in different subclones. Despite having these features in common, differences in antigen expression were noted between the two cell lines: that of MOLM-13 being CD34+, CD13-, CD14-, CD15+, CD33+; whereas MOLM-14 was CD4+, CD13+, CD14+, CD15+, CD33+. Differentiation to macrophage-like morphology could be induced in both cell lines after stimulation with INF-gamma alone, or in combination with TNF-alpha, which treatment also induced or upregulated, expression of certain myelomonocyte-associated antigens, including CD13, CD14, CD15, CD64, CD65 and CD87. Together, these data confirm that both cell lines are likely to be novel in vitro models for studying monocytic differentiation and leukemogenesis.

更新日期：2019-11-01

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