A fluorescent analog of cis-diamminedichloroplatinum(II), cis-bis(6-aminoquinoline)dichloroplatinum(II), was prepared from K2[PtCl4] and 6-aminoquinoline (AQL). HPLC of the thiourea derivative of the new complex showed that it has the cis-configuration. The Pt-AQL complex and the parent ligand AQL were evaluated for biological activity and cellular uptake, using the ciliate Tetrahymena pigmentosa. The complex was relatively nontoxic at the tested levels below 5 x 10(-4) M, but did exhibit inhibition of culture growth at 5 x 10(-4) M. Measurement of cellular uptake of the Pt-AQL complex demonstrated incorporation into the cell, with localization primarily within the vacuoles of the cells. Comparable measurement of the parent ligand AQL showed little measurable cellular uptake.

中文翻译：

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顺式双（6-氨基喹啉）二氯铂（II）的细胞吸收和毒性研究。

从K2 [PtCl4]和6-氨基喹啉（AQL）制备了顺式二甲基二氯铂（II）的荧光类似物，即顺式双（6-氨基喹啉）二氯铂（II）。新的配合物的硫脲衍生物的HPLC显示其具有顺式构型。使用纤毛四膜虫评估了Pt-AQL复合物和母体配体AQL的生物活性和细胞摄取。在低于5 x 10（-4）M的测试水平下，该复合物相对无毒，但在5 x 10（-4）M时却显示出对培养物生长的抑制作用。对Pt-AQL复合物细胞摄取的测量表明，该复合物可掺入细胞，主要定位在细胞的液泡内。母体配体AQL的可比测量显示几乎没有可测量的细胞摄取。