ABI1和ABI2蛋白磷酸酶2C在脱落酸信号通路的负反馈调节回路中起作用。,The Plant Journal

当前位置： X-MOL 学术 › Plant J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

ABI1和ABI2蛋白磷酸酶2C在脱落酸信号通路的负反馈调节回路中起作用。
The Plant Journal ( IF 6.2 ) Pub Date : 2001-02-24 , DOI: 10.1046/j.1365-313x.2001.00965.x
S Merlot ₁ , F Gosti , D Guerrier , A Vavasseur , J Giraudat

Affiliation

拟南芥ABI1和ABI2基因编码两个蛋白丝氨酸/苏氨酸磷酸酶2C（PP2C）。这些基因最初是由显性突变abi1--1和abi2--1鉴定的，这些突变降低了植物对脱落酸（ABA）的反应能力。但是，最近发现ABI1的隐性突变体对ABA敏感，这表明ABI1磷酸酶是ABA信号的负调节剂。我们在此报告了ABI2第一个功能降低的等位基因abi2--1R1的分离和表征。abi2--1R1蛋白的体外磷酸酶活性比野生型ABI2蛋白的体外磷酸酶活性低约100倍。Abi2--1R1植物表现出野生型ABA敏感性。然而，与每个亲本单个突变体相比，在ABI1基因座处结合了abi2--1R1等位基因和功能丧失等位基因的双突变体植物对ABA的响应更高。这些数据表明，野生型ABI2磷酸酶是ABA信号的负调节剂，并且ABI1和ABI2磷酸酶在控制ABA作用中具有重叠的作用。测量植物提取物中的PP2C活性表明，ABI1和ABI2的磷酸酶活性响应于ABA而增加。这些结果表明，ABI1和ABI2在ABA信号通路的负反馈调节回路中起作用。测量植物提取物中的PP2C活性表明，ABI1和ABI2的磷酸酶活性响应于ABA而增加。这些结果表明，ABI1和ABI2在ABA信号通路的负反馈调节回路中起作用。测量植物提取物中的PP2C活性表明，ABI1和ABI2的磷酸酶活性响应于ABA而增加。这些结果表明，ABI1和ABI2在ABA信号通路的负反馈调节回路中起作用。

"点击查看英文标题和摘要"

The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway.

The Arabidopsis ABI1 and ABI2 genes encode two protein serine/threonine phosphatases 2C (PP2C). These genes have been originally identified by the dominant mutations abi1--1 and abi2--1, which reduce the plant's responsiveness to the hormone abscisic acid (ABA). However, recessive mutants of ABI1 were recently shown to be supersensitive to ABA, which demonstrated that the ABI1 phosphatase is a negative regulator of ABA signalling. We report here the isolation and characterisation of the first reduction-of-function allele of ABI2, abi2--1R1. The in vitro phosphatase activity of the abi2--1R1 protein is approximately 100-fold lower than that of the wild-type ABI2 protein. Abi2--1R1 plants displayed a wild-type ABA sensitivity. However, doubly mutant plants combining the abi2--1R1 allele and a loss-of-function allele at the ABI1 locus were more responsive to ABA than each of the parental single mutants. These data indicate that the wild-type ABI2 phosphatase is a negative regulator of ABA signalling, and that the ABI1 and ABI2 phosphatases have overlapping roles in controlling ABA action. Measurements of PP2C activity in plant extracts showed that the phosphatase activity of ABI1 and ABI2 increases in response to ABA. These results suggest that ABI1 and ABI2 act in a negative feedback regulatory loop of the ABA signalling pathway.

更新日期：2019-11-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文