From a CH2Cl2 extract of the Mediterranean sponge Anchinoe paupertas were isolated and characterized zarzissine [2], a new 4,5-guanidino-pyridazine compound and the known p-hydroxybenzaldehyde [1]. The structure of zarzissine [2] was elucidated by spectroscopic methods, including the application of a number of 2D nmr techniques. Biological activities of compounds 1 and 2 were also determined, with zarzissine exhibiting cytotoxicity against three human and murine tumor cell lines.

中文翻译：

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Zarzissine，一种来自地中海海绵Anchinoe paupertas的新型细胞毒性胍生物碱。

从地中海海绵的CH2Cl2提取物中分离了An（Anchinoe paupertas）并对其进行了表征：查尔济辛[2]，新的4,5-胍基-哒嗪化合物和已知的对羟基苯甲醛[1]。通过光谱方法阐明了zarzissine [2]的结构，包括许多2D nmr技术的应用。还测定了化合物1和2的生物活性，其中zarzissine对三种人类和鼠类肿瘤细胞系均表现出细胞毒性。