人类CD26（二肽基肽酶IV）基因的基因组组织，确切定位和组织表达。,Immunogenetics

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人类CD26（二肽基肽酶IV）基因的基因组组织，确切定位和组织表达。
Immunogenetics ( IF 2.9 ) Pub Date : 1994-01-01 , DOI: 10.1007/bf01246674
C A Abbott ₁ , E Baker , G R Sutherland , G W McCaughan

Affiliation

CD26是一种淋巴细胞表面抗原，在T细胞活化过程中会增加，并在其他组织中表达。它是属于脯氨酰寡肽酶家族的非典型丝氨酸蛋白酶。CD26与多种生物学功能有关，包括T细胞活化，细胞间粘附以及最近与HIV感染有关。本文通过八个人类CD26基因组克隆的分离和部分测序，描述了脯氨酰寡肽酶家族的基因组组织的第一个信息。我们已经确定，人类CD26基因跨越大约70千个碱基（kb），并包含26个外显子，大小从45个碱基对（bp）到1.4 kb不等。编码丝氨酸识别位点（GWSYG）的核苷酸在两个外显子之间分裂。这清楚地将脯氨酰寡肽酶家族的基因组组织与经典丝氨酸蛋白酶家族的组织学区别开。CD26基因的5'侧翼结构域既不包含TATA框也不包含CAAT框，但是一个非常丰富的C和G（72％）的300 bp区域包含一些转录因子的潜在结合位点。人类CD26基因编码两条消息，大小分别约为4.2和2.8 kb。这些都在胎盘和肾脏中高水平表达，在肺和肝中中等水平表达。在骨骼肌，心脏，大脑和胰腺中仅以低水平表达4.2 kb mRNA。中期染色体扩散的荧光原位杂交将人类CD26基因定位于2号染色体的长臂（2q24.3）。CD26基因的侧翼结构域既不包含TATA框，也不包含CAAT框，但是一个极富C和G的300 bp区域（72％）包含一些转录因子的潜在结合位点。人类CD26基因编码两条消息，大小分别约为4.2和2.8 kb。这些都在胎盘和肾脏中高水平表达，在肺和肝中中等水平表达。在骨骼肌，心脏，大脑和胰腺中仅以低水平表达4.2 kb mRNA。中期染色体扩散的荧光原位杂交将人类CD26基因定位于2号染色体的长臂（2q24.3）。CD26基因的侧翼结构域既不包含TATA框，也不包含CAAT框，但是一个极富C和G的300 bp区域（72％）包含一些转录因子的潜在结合位点。人类CD26基因编码两条消息，大小分别约为4.2和2.8 kb。这些都在胎盘和肾脏中高水平表达，在肺和肝中中等水平表达。在骨骼肌，心脏，大脑和胰腺中仅以低水平表达了4.2 kb的mRNA。中期染色体扩散的荧光原位杂交将人类CD26基因定位于2号染色体的长臂（2q24.3）。人类CD26基因编码两条消息，大小分别约为4.2和2.8 kb。它们均在胎盘和肾脏中高水平表达，在肺和肝脏中中等水平表达。在骨骼肌，心脏，大脑和胰腺中仅以低水平表达4.2 kb mRNA。中期染色体扩散的荧光原位杂交将人类CD26基因定位于2号染色体的长臂（2q24.3）。人类CD26基因编码两条消息，大小分别约为4.2和2.8 kb。它们均在胎盘和肾脏中高水平表达，在肺和肝脏中中等水平表达。在骨骼肌，心脏，大脑和胰腺中仅以低水平表达4.2 kb mRNA。中期染色体扩散的荧光原位杂交将人类CD26基因定位于2号染色体的长臂（2q24.3）。

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Genomic organization, exact localization, and tissue expression of the human CD26 (dipeptidyl peptidase IV) gene.

CD26 is a lymphocyte cell surface antigen which is increased during T-cell activation and is also expressed in other tissues. It is an atypical serine protease belonging to the prolyl oligopeptidase family. CD26 has been implicated in a variety of biological functions including T-cell activation, cell-to-cell adhesion, and recently in HIV infection. This paper describes, through the isolation and partial sequencing of eight human CD26 genomic clones, the first information on the genomic organization of the prolyl oligopeptidase family. We have established that the human CD26 gene spans approximately 70 kilobases (kb) and contains 26 exons, ranging in size from 45 base pairs (bp) to 1.4 kb. The nucleotides that encode the serine recognition site (G-W-S-Y-G) are split between two exons. This clearly distinguishes the genomic organization of the prolyl oligopeptidase family from that of the classical serine protease family. The 5' flanking domain of the CD26 gene contains neither a TATA box nor a CAAT box, but a 300 bp region extremely rich in C and G (72%) contains potential binding sites for several transcriptional factors. The human CD26 gene encodes two messages sized at about 4.2 and 2.8 kb. These are both expressed at high levels in the placenta and kidney and at moderate levels in the lung and liver. Only the 4.2 kb mRNA was expressed at low levels in skeletal muscle, heart, brain, and pancreas. Fluorescence in situ hybridization on metaphase chromosome spreads located the human CD26 gene to the long arm of chromosome 2(2q24.3).

更新日期：2019-11-01

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