水稻β-葡聚糖酶基因的结构受乙烯，细胞分裂素，创伤，水杨酸和真菌激发子调控。,Plant Molecular Biology

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水稻β-葡聚糖酶基因的结构受乙烯，细胞分裂素，创伤，水杨酸和真菌激发子调控。
Plant Molecular Biology ( IF 3.9 ) Pub Date : 1992-01-01 , DOI: 10.1007/bf00018454
C R Simmons ₁ , J C Litts , N Huang , R L Rodriguez

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对水稻β-葡聚糖酶基因进行测序，并在mRNA积累水平分析其表达。该基因（Gns1）在发芽的种子，芽，叶，穗和愈伤组织中的表达水平相对较低，而在根中则表达水平较高。根中的表达似乎是组成性的。当用乙烯，细胞分裂素，水杨酸和源自病原菌米氏菌或非病原性酿酒酵母的真菌引发剂处理时，芽表达的Gns1含量要高得多。芽也响应受伤而以更高的水平表达Gns1。生长素，赤霉素或脱落酸对芽中的表达没有显着影响。β-葡聚糖酶与大麦1,3;1,4-β-D-葡聚糖酶显示82％的氨基酸相似性，从杂交研究来看，它是水稻基因组中最接近大麦1,3;1,4-β-葡聚糖酶EI基因的β-葡聚糖酶基因。成熟的肽具有32kDa的计算分子量。该基因在信号肽第25个氨基酸的密码子中具有3145 bp的大内含子。该基因在成熟肽编码区的密码子第三位表现出非常强的密码子偏倚，即99％G + C，但在信号肽区中只有61％G +C。

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Structure of a rice beta-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors.

A rice beta-glucanase gene was sequenced and its expression analyzed at the level of mRNA accumulation. This gene (Gns1) is expressed at relatively low levels in germinating seeds, shoots, leaves, panicles and callus, but it is expressed at higher levels in roots. Expression in the roots appears to be constitutive. Shoots express Gns1 at much higher levels when treated with ethylene, cytokinin, salicylic acid, and fungal elicitors derived from the pathogen Sclerotium oryzae or from the non-pathogen Saccharomyces cereviseae. Shoots also express Gns1 at higher levels in response to wounding. Expression in the shoots is not significantly affected by auxin, gibberellic acid or abscisic acid. The beta-glucanase shows 82% amino acid similarity to the barley 1,3;1,4-beta-D-glucanases, and from hybridization studies it is the beta-glucanase gene in the rice genome closest to the barley 1,3;1,4-beta-glucanase EI gene. The mature peptide has a calculated molecular mass of 32 kDa. The gene has a large 3145 bp intron in the codon for the 25th amino acid of the signal peptide. The gene exhibits a very strong codon bias of 99% G + C in the third position of the codon in the mature peptide coding region, but only 61% G + C in the signal peptide region.

更新日期：2019-11-01

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