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A calfluxin-related peptide is present in the bag cells and atrial gland of Aplysia.
General and Comparative Endocrinology ( IF 2.1 ) Pub Date : 1990-07-01 , DOI: 10.1016/0016-6480(90)90094-3
M de Jong-Brink 1 , G T Nagle , W J Dictus , S D Painter , T Broers-Vendrig , J E Blankenship
Affiliation  

The caudodorsal cells of Lymnaea and the bag cells of Aplysia are neuroendocrine cells whose peptide products have homologous functions, i.e., regulation of egg deposition. One of the Lymnaea products, calfluxin, increases cytosolic and hence mitochondrial calcium concentrations in secretory cells of the albumen gland, an exocrine organ that secretes perivitellin fluid around the egg cells during packaging; the changes can be visualized at the ultrastructural level for quantification with the pyroantimonate precipitation technique and are correlated with changes in the secretory and biosynthetic activity in the gland. Comparable studies have now been carried out with Aplysia and indicate that the bag cells of A. californica and A. brasiliana also contain a factor with calfluxin-related activity, and that the factor is not the egg-laying hormone (ELH). The bag cell factor does not affect mitochondrial calcium levels in the Lymnaea albumen gland, and synthetic calfluxin does not affect the Aplysia gland. Thus, although the bag cell and caudodorsal cell peptides have the same activity in their respective genera, the sequences have diverged sufficiently during the course of evolution to preclude cross-reactivity. Calfluxin-related activity was also detected in the atrial gland of A. californica and the atrial gland-like epithelium of A. brasiliana, two exocrine organs in the oviduct that express genes structurally related to the bag cell ELH gene. It is postulated that the active atrial gland factors may be peptides A and B.

中文翻译:

钙氟蛋白相关肽存在于海藻的袋细胞和心房腺中。

lymnaea的尾鳍细胞和Aplysia的袋细胞是神经内分泌细胞,其肽产物具有同源功能,即调节卵的沉积。Lymnaea的一种产物,钙氟蛋白,增加了蛋白腺分泌细胞中胞质钙的浓度,从而增加了线粒体钙的浓度。蛋白分泌腺是一种外分泌器官,在包装过程中会在卵细胞周围分泌卵白蛋白。这些变化可以通过焦锑酸盐沉淀技术在超微结构水平上进行可视化量化,并与腺体分泌和生物合成活性的变化相关。现在已对Aplysia进行了可比的研究,这些研究表明,加利福尼亚州立农杆菌和巴西拟南芥的袋状细胞还含有与钙氟蛋白相关活性的因子,并且该因素不是产卵激素(ELH)。袋细胞因子不影响Lymnaea蛋白腺中的线粒体钙水平,而合成的钙氟蛋白不影响海葵腺。因此,尽管袋细胞和尾嗅细胞肽在它们各自的属中具有相同的活性,但是这些序列在进化过程中已经充分分开以防止交叉反应。在加利福尼亚州的A. californica的心房和巴西的A. brasiliana的心房样上皮中也检测到了钙氟蛋白相关的活性,这两个输卵管在输卵管中表达的结构与袋细胞ELH基因相关。假定活性心房腺因子可能是肽A和B。合成的钙氟蛋白不影响海藻腺。因此,尽管袋细胞和尾嗅细胞肽在它们各自的属中具有相同的活性,但是这些序列在进化过程中已经充分分开以防止交叉反应。在加利福尼亚州的A. californica的心房和巴西的A. brasiliana的心房样上皮中也检测到了钙氟蛋白相关的活性,这两个输卵管在输卵管中表达的结构与袋细胞ELH基因相关。假定活性心房腺因子可能是肽A和B。合成的钙氟蛋白不影响海藻腺。因此,尽管袋细胞和尾嗅细胞肽在它们各自的属中具有相同的活性,但是这些序列在进化过程中已经充分分开以防止交叉反应。在加利福尼亚州的A. californica的心房和巴西的A. brasiliana的心房样上皮中也检测到了钙氟蛋白相关的活性,这两个输卵管在输卵管中表达的结构与袋细胞ELH基因相关。假定活性心房腺因子可能是肽A和B。在加利福尼亚州的A. californica的心房和巴西的A. brasiliana的心房样上皮中也检测到了钙氟蛋白相关的活性,这两个输卵管在输卵管中表达的结构与袋细胞ELH基因相关。假定活性心房腺因子可能是肽A和B。在加利福尼亚州的A. californica的心房和巴西的A. brasiliana的心房样上皮中也检测到了钙氟蛋白相关的活性,这两个输卵管在输卵管中表达的结构与袋细胞ELH基因相关。假定活性心房腺因子可能是肽A和B。
更新日期:2019-11-01
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